摘要
目的证实水通道蛋白5(aquaporin 5, AQP5)在实验性变应性鼻炎大鼠鼻黏膜中的表达及分布,并探讨其与变应性鼻炎的关系.方法挑选健康Wistar大鼠24只,雌雄不限,体重200～300 g,随机分为实验组和对照组各12只.实验组用卵白蛋白行基础致敏,继之鼻腔局部激发建立大鼠变应性鼻炎模型;对照组用生理盐水代替卵白蛋白进行试验.取所有大鼠的鼻黏膜,应用免疫荧光技术定位AQP5在对照组和实验组大鼠鼻黏膜中的分布,并采用免疫组化技术进一步检测AQP5在两组大鼠鼻黏膜中的表达.结果①实验组大鼠鼻黏膜常规HE染色表现为明显的腺体增生、炎症反应和嗜酸粒细胞浸润;②免疫组化染色及免疫荧光技术均显示AQP5在实验组和对照组大鼠鼻黏膜中的分布基本一致,主要表达在腺上皮细胞、导管上皮细胞及纤毛上皮细胞的胞膜和胞质中;③免疫组化染色的统计学分析显示,实验组AQP5在黏膜细胞中表达的量(156.37±1.93)明显高于对照组(178.52±1.94),两组间差异有统计学意义(t=28.08, P<0.01).结论变应性鼻炎时,腺体过度分泌与AQP5的高表达密切相关.
Objective To confirm the expression and distribution of aquaporin 5 (AQP5) in rat nasal mucosa of experimental allergic rhinitis and to investigate the relationship between AQP5 and allergic rhinitis. Methods Twenty-four healthy Wistar rats both male and female weighting 200-300 g were divided into two groups randomly, one was testing group (n=12), the other was comparing group(n=12). Generally sensitized rats in testing group were given repeated local booster sensitization into nasal cavity. Twelve normal rat nasal mucosa and twelve nasal mucosa from rat with allergic rhinitis were used. The distribution of AQP5 in normal rat nasal mucosa and nasal mucosa in rat with allergic rhinitis were observed by immunofluorescence technique. Furthermore, the expression of AQP5 in normal rat nasal mucosa and nasal mucosa from rat with allergic rhinitis were studied by immunohistochemical staining. Results ①Hematoxylin and eosin staining showed that there was obvious inflammation reaction, a large quantity of glands hyperplasia and acidophils soaked in nasal mucosa from rat with allergic rhinitis.②Both immunofluorescence technique and immunohistochemical staining showed that the distribution of AQP5 in normal rat nasal mucosa was in accordance with that in nasal mucosa from rat with allergic rhinitis on the whole.AQP5 expressed mainly in the membrane and cytoplasm of the epithelium in the glands, ducts and cilia.③The statistical analysis of the immunohistochemical staining showed that the quantity of AQP5 in rat nasal mucosa with allergic rhinitis (156.37±1.93) was obviously higher than that in the normal rat nasal mucosa (178.52±1.94). There was statistical significance between the two groups(t=28.08, P<0.01). Conclusions The hypersecretion of glands has a close relationship with the high expression of AQP5 in allergic rhinitis.
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2005年第3期172-175,共4页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
