摘要
对超数排卵的小鼠分别在注射HCG后14～16,16～18,18～20和20～22 h采小鼠卵母细胞用于核移植,观察小鼠卵母细胞的去核时间在体细胞核移植中的作用.结果显示,在注射hCG后14～16,16～18,18～20和20～22 h,小鼠卵母细胞去核效率分别为80.09%(338/422),73.12%(283/387),55.02%(230/418)和48.74%(193/396);重构胚激活率分别为66.20%(190/287),75.43%(175/232),84.78%(156/184)和85.14%(126/148);重构胚的囊胚发育率分别为13.94%(40/287),7.33%(17/232),3.80%(7/184)和1.35%(2/148).试验证实小鼠卵母细胞在注射hCG后14～16 h进行去核较为适宜.
Mice oocytes were enucleated during 14~16, 16~18, 18~20 and 20~22 h apart after injected hCG (Human Chorionic Gondaotropin, hCG), so as to research effect on mice somatic cell nucear transfer about enucleation time. Results show that encleation rate of mice oocytes was 80.09 %(338/422), 73.12 %(283 / 387), 55.02 %(230/418), and 48.74 %(193/396) respectively; Activation rate of embryos reconstructed was 66.20 %(190/287), 75.43 %(175 /232), 84.78 %(156/184), 85.14 %(126/148) apart; Blastocyst rate of embryos reconstructed was 13.94 %(40/287), 7.33 %(17/232), 3.80 %(7/184) and 1.35 %(2/148) respectively. It is indicated that enucleation time of mice oocytes is good during 14~16 h after injected hCG.
出处
《中国农学通报》
CSCD
2005年第3期23-26,共4页
Chinese Agricultural Science Bulletin
基金
国家"863"高技术项目"家畜体细胞克隆技术体系的建立和应用"(编号:2001AA213081)。
