摘要
背景:脑血管疾病有多种发病机制,自由基及其脂质过氧化作用参与了脑缺血后神经细胞的损害过程。补阳还五汤是一种临床上常用的治疗缺血性脑血管病中药,其作用机制还有许多不明之处。目的:观察补阳还五汤及拆方对脑缺血再灌注模型大鼠血清及脑组织超氧化物歧化酶(SOD)、丙二醛及一氧化氮含量影响,探讨其作用机制,并研究方剂配伍的意义。设计:以实验动物为研究对象,随机对照的实验研究。单位:一所中医学院医院的神经内科。材料:实验在河南省中医研究院国家中医管理局三级实验室进行研究,选择上海西普尔-必凯实验动物有限公司提供的清洁级SD大鼠60只。干预:60只大鼠随机分为假手术组、模型组、活血组、黄芪组、总方组。通过四血管结扎法制作SD大鼠脑缺血再灌注模型,分别给予活血组药物8g/kg、黄芪组药物40g/kg、总方组药物48g/kg,假手术组、模型组给予生理盐水20mL/kg。取血清及脑组织匀浆应用黄嘌呤氧化酶法测定SOD,用硫代巴比妥酸法测定丙二醛。采用硝酸还原酶法测定一氧化氮的含量。主要观察指标:各组大鼠血清、脑组织超氧化物歧化酶(SOD)、丙二醛及一氧化氮含量。结果:模型组大鼠血清、脑组织丙二醛及一氧化氮含量明显增高(P<0.001～0.05),SOD含量明显降低(P<0.05,P<0.01);总方组与拆方黄芪组、活血组?
BACKGROUND:There are various mechanisms on the occurrence of cerebral vascula r disorders,in which,free radical and lipid peroxidation have participated in th e process of nerve cell injury after cerebral ischemia.Buyang huanwu tang is a k ind of commonly used Chinese herb for the treatment of ischemic cerebral vascula r disorders, but its mechanisms have been unknown.OBJECTIVE:To observe effect of buyang huanwu tang and its disassemble formula s on the levels of superoxide dismutase(SOD), malondialdehyde(MAD) and nitric ox ide(NO) in serum and brain tissue in cerebral ischemic reperfusion model in rats ,probe into its mechanisms and study on the significance of formula compatibilit y.DESIGN:Experimental study with randomized control based on experimental anima ls.SETTING:Neurological internal department of one hospital affiliated to an ins titution of Chinese medicine.MATERIALS:The experiment was performed in 3rd Grade Experimental Room of Sta te Administration of Traditional Chinese Medicine,Henan Academy of Chinese Medic ine.Sixty clean- grade SD rats were employed in the experiment,provided by Shan ghai SIPPR/BK Experimental Animal Co. Ltd.INTERVENTIONS:Sixty rats were randomized into sham- operation group,model gr oup,huangqi(Radix Astragali seu Hedysari) group and general formula group.The mo del of cerebral ischemic reperfusion in SD rats were prepared by four vessel lig ature method, in which, the medicine was applied at the dosages of 8 g/kg,40 g/k g and 48 g/kg in blood activation group, huangqi(Radix Astragali seu Hedysari) g roup and general formula group successively;and physiological saline 20 mL/kg wa s applied in both sham- operation group and model group. Serum and homogenate i n brain tissue were collected to assay SOD with xanthine oxidase method,MAD with thiobarbituric acid method and NO with nitrate reductase method.MAIN OUTCOME MEARSURES:Levels of SOD,MAD and NO in serum and brain tissue of rats in every group.RESULTS:The levels of MAD and NO in serum and brain tissue in model group wer e remarkably increased(P< 0.001- 0.05) and SOD was remarkably decreased(P< 0.05 ,P< 0.01).In general formula group,huangqi(Radix Astragali seu Hedysari) group a nd blood activation group of disassemble formulas,the levels of MAD and NO were reduced and SOD was increased,for which,the action of general formula group was the strongest(P< 0.01).CONCLUSION:Buyang huanwu tang and disassemble formulas act on being against t he cerebral ischemic reperfusion injury achieved by anti- free radical.
出处
《中国临床康复》
CSCD
北大核心
2005年第9期237-239,共3页
Chinese Journal of Clinical Rehabilitation
基金
河南省自然基金(0211042100)~~
