摘要
建立了用ＨＰＬＣ柱切换测定血浆中氟康唑的方法。血样用０．２ｍｏｌ·Ｌ－１醋酸溶液简单稀释后注入填充ＬｉＣｈｒｏｍｐｒｅｐＲＰ２（２５～４０μｍ）的预柱上，用蒸馏水冲洗出血浆中蛋白和其它极性成分。切换后，浓缩在预柱上的氟康唑被流动相甲醇－０．２ｍｏｌ·Ｌ－１醋酸按（ｐＨ２．７）（５０：５０）反冲到分析柱ＳｈｉｍｐａｃｋＣＬＣ－ＯＤＳ上进行分析。预柱用净化液进行清除和再生。本法有很好的精密度与回收率，检测限为０．１２μｇ·ｍｌ－１血浆，日间和日内测定相对标准偏差均小于６％。此分析法已成功地用于测定自愿受试者的氟康唑药代动力学及生物利用度。
High performance liquid chromatography with colunm switching has been
developed for the determination of fluconazole in plasma.The plasma
samples were injected onto a precolumn packed with LiChromprep
RP2(25～40μm)after simple dilution with a solution of 0.2
mol·L-1 acetic acid.Distilled water was used to wash out protein and
other polar components in plasma.After switching, the concentrated
fluconazole was eluted in the back-flush mode onto a Shimpack CLC-ODS
column with methanol-0.2 mol·L-1 ammonium acetate(pH 2.7)(50 :50 )as
mobile phase.Purge solutions were used for clean-up and for
regenerating the precolumn,The method showed a good precision and
excellent recovery.The detection limit was 0.12μg·ml-1 plasma.The
RSD's(intra-andinterday )were less than 6.3%. The method has been
successfully used to determine bioavailability and pharmacokinetics
of fluconazole in Chinese volunteers.
出处
《药学学报》
CAS
CSCD
北大核心
1994年第10期773-777,共5页
Acta Pharmaceutica Sinica
关键词
氟康唑
药代动力学
高效液相色谱
Fluconazole
Pharmacokinetics
Bioavailability
HPLC
Column switching
technique
