摘要
以表面带羟基的磁性高分子微球为载体，对位苯醌活化后，通过共价结合修饰中性蛋白酶，得到比活性为１０００Ｕ／ｇ的磁性固定化酶。偶联蛋白量２０～３０ｍｇ／ｇ载体，固定化酶活性保持达４０％，自由酶和固定化酶相比，最适温度从５０℃变到５０～６０℃，最适ｐＨ从７．５变到６．５，Ｋｍ从０．０５４％变到０．０８８％酪蛋白溶液，ｐＨ稳定性、热稳定性、贮存稳定性都有较大提高．
A neutral protease from Balilius Subtilis has been immobilized onto the magneticpolymer microspheres,which were obtained from styrene,hydroxyethyl methacrylate,di-vinyl benzene and Fe_3O_4,and had hydroxyl groups in the surface by covalent coupling.Themagnetic immobilized neutral protease had a enzyme activity of 1000U/g,enzyme yield of20～30mg/g carrier,and activity retention of about 40%.For both free enzyme and immobi-lized enzyme the optimum work temperatures were 50℃ and 50～60℃,the optimum pHvalues were 7.5 and 6.5, and the kinetic constants were 0.054%and 0.088%,respectively.The thermal stability, pH stability,and the storage stability of the immobilized enzyme areimproved obviously.
出处
《应用化学》
CAS
CSCD
北大核心
1994年第5期1-4,共4页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金资助项目
关键词
高分子微球
固定化
中性蛋白酶
magnetic polymer microsphere,neutral protease,immobilization
