摘要
桑皱褶花叶病是一种由螺原体寄生引起的桑树病害，通过嫁接，昆虫及汁液传病，近年在浙江省局部地区蔓延危害。病原螺原体在革兰氏染色反应中呈阳性反应，能在植物性液体培养基和固体培养基中培养成功。螺原体形态有圆形、椭圆形、螺旋形和线状等。圆形粒体直径为８３～７００ｎｍ，基本螺旋体大小为３６４～８３３ｎｍ×８２～１１９ｎｍ，线状体长为３．０～４．５μｍ，宽为１００ｎｍ。本病原寄主范围较广。土霉素（２５０～５００ｍｇ／ｋｇ）对本病具有明显的抑制作用。本病原螺原体的生活力及致病性均强，经液体培养１ａ后，能对无病实生苗传毒成功。
Mulberry mosaic disease was found to be caused by a spiroplasmaand distributed in several places of Zhejiang.This disease could be trans-mitted directiy through grafting,insects, and could be inoculated by mecha-nical method on to some mulberry seedlings,The crinkle spiroplasma whichshowed positive reaction with gram-staining,and could be cultured in liquidmedium and Solid medium, was still living after one year.The morphologyof crinkle spiroplasma for sizes was 364～833 nm × 82～119 nm(elementalyhelix),and 83～700 nm in diameter(spherical bodies),or 3.0～4.5μm inlength and 100 nm in width (myciliod).The host range of crinkle spiro-plasma was wide. Theraputic tests using oxytetracycline at 250～500 mg/kgspraying on to diseased mulberry plants resulted in effective control of thedisease.
出处
《浙江林学院学报》
CSCD
1994年第2期171-176,共6页
Journal of Zhejiang Forestry College
关键词
桑树
花叶病
离体培养
mulberries
spiroplasma disease
mosaic disease
oxytetracycline
culture in vitro
