摘要
目的 研究人参皂苷 Ro对小鼠脾细胞增殖及细胞因子产生的影响。方法 [3H]TdR参入法检测人参皂苷 Ro对小鼠脾淋巴细胞增殖的影响;酶联免疫吸附法检测人参皂苷 Ro对小鼠脾淋巴细胞产生细胞因子白介素 2、干扰素 γ和白介素 4的影响;逆转录聚合酶链式反应分析法研究人参皂苷 Ro对小鼠脾淋巴细胞中干扰素 γ、白介素 4mRNA表达的影响。结果 人参皂苷 Ro在 1-10μmol·L-1显著促进ConA诱导的小鼠脾淋巴细胞增殖及小鼠脾淋巴细胞白介素 2的产生;在 2-10μmol·L-1促进ConA诱导的小鼠脾淋巴细胞产生和表达Th2细胞因子白介素 4, 而降低ConA诱导的小鼠脾淋巴细胞产生和表达Th1细胞因子干扰素 γ。结论 人参皂苷 Ro通过调节脾细胞内Th1型和Th2型细胞因子的转录和表达发挥免疫调节作用。
Aim To study the effects of ginsenoside-Ro on cell proliferation and cytokine production in murine splenocytes. Methods The effect of ginsenoside-Ro on murine splenocytes proliferation was studied using [ 3H]thymidine incorporation assay. Effects of ginsenoside-Ro on the production of cytokines interleukin-2 (IL-2), interferon-γ (IFN-γ) and interleukin-4 (IL-4) from murine splenocytes were detected by ELISA method. Effects of ginsenoside-Ro on mRNA level of Th1 cytokine IFN-γ and Th2 cytokine IL-4 were evaluated by reverse transcription polymerase chain reaction (RT-PCR) analysis. Results Ginsenoside-Ro showed no mitogenic effect on unstimulated murine splenocytes. It enhanced the proliferation of Con A-induced murine splenocytes and the production of IL-2 at concentrations of 1-10 μmol·L -1. Moreover, ginsenoside-Ro increased the production and expression of Th2 cytokine IL-4 and decreased the production and expression of Th1 cytokine IFN-γ in Con A-induced murine splenocytes at concentrations of 2-10 μmol·L -1. Conclusion Ginsenoside-Ro showed immunomodulatory effects by regulating the production and expression of Th1/Th2 cytokines in murine splenocytes.
出处
《药学学报》
CAS
CSCD
北大核心
2005年第4期332-336,共5页
Acta Pharmaceutica Sinica
