摘要
目的探讨江苏省淋病奈瑟菌(NG)对氟喹诺酮的耐药状况、耐药基因突变型的分布情况及二者之间的关系。方法采用琼脂稀释法检测江苏省三个地区临床分离的95株NG氟喹诺酮类药物的最小抑菌浓度(MIC),根据结果抽取部分菌株采用PCR法扩增gyrA基因的氟喹诺酮耐药决定区(QRDR),并进行测序分析。结果根据所测MIC,95株NG对环丙沙星100%的耐药;60株通过测序分析,检测到gyrA的五种突变形式,以gyrA出现272位碱基C→T和283位G→A的突变型的MIC最高。结论在江苏省,NG对氟喹诺酮耐药严重,氟喹诺酮药物已不再适合用于临床治疗淋病。
Objective To explore the status and the distribution of gene mutation of N.gonorrhoeae resistance to fluoroquinolones in jiangsu province.Methods Antimicrobial susceptibilities of 95 N.gonorrhoeae isolated in STD clinics were determined by agar dilution method,according to the results of susceptibilities,gyrA gene of fluoroquinolones resistant Gonococcus and a part of susceptible strains were amplified by PCR and further examined by sequence analysis,the mutations were identified by comparison to ATCC19424 strain’s gyrA sequence.Results The results showed that the fluoroquinolons resistance in gonorroheae was 100% in Jiangsu province.Five kinds of mutation types of gyrA gene were detected in 60 isolates.Conclusion Quinolone are not effective for treatment of N.gonorrhoeae and continued monitoring of resistance is essential in Jiangsu.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2005年第4期193-195,共3页
The Chinese Journal of Dermatovenereology
基金
江苏省卫生厅预防基金资助(项目编号:Y200408)
