摘要
目的 通过体外实验评价紫杉醇对人胰腺癌细胞Bxpc - 3的抑制作用。 方法 应用形态学、MTT分析法、流式细胞术对紫杉醇诱导的人胰腺癌细胞Bxpc - 3进行检测和观察。结果 人胰腺癌细胞Bxpc - 3在紫杉醇的作用下 ,细胞发生一系列形态学改变 ;紫杉醇对Bxpc - 3的抑制率有剂量和时间的依赖关系 ;流式细胞仪分析结果显示细胞有G2 -M期阻滞和细胞凋亡。结论 紫杉醇诱发的人胰腺癌细胞凋亡与细胞周期阻滞密切相关 ,随浓度和时间的延长 ,G2 -M期细胞的比例增高。
Objective To assess the inhibition of paclitaxol on human pancreatic cancer Bxpc-3 cell in vitro. Methods Cell morphology, MTT assay and flow cytometry were used to examine paclitaxol-induced apoptosis and growth inhibition in human pancreatic cancer Bxpc-3 cell. Results Cells treated with paclitaxol have much changes in morphology. The growth inhibition effect of paclitaxol on Bxpc-3 cells was time and dosage dependent. The result of flow cytometry shows cells were blocked in G 2-M phase and were induced to apoptosis. Conclusions Paclitaxol can block Bxpc-3 cell in G 2-M phase, the induction of apoptosis Bxpc-3 cell by Paclitaxol was closely associated with mitotic arrest of cell cycle.
出处
《锦州医学院学报》
2005年第1期15-17,共3页
Journal of Jinzhou Medical College
关键词
紫杉醇
胰腺癌
凋亡
Paclitaxel
pancreatic cancer
apoptosis
