摘要
目的 探讨乳腺癌的微卫星不稳定性及其与临床资料的关系。方法 选取6个微卫星位点及4 0例正常组织和其对应的自身肿瘤对照组织,提取DNA后用PCR扩增,电泳,银染显色后进行微卫星不稳定性分析。用免疫组化观察p5 3、PR、ER在乳腺癌中表达情况。结果 在4 0例乳腺癌中有9例至少1个位点出现MSI。MSI和病人年龄、肿瘤大小、p5 3之间没有明显的相关性。但ER、PR阴性和淋巴结转移的病例出现MSI的比例远远高于ER和PR阳性病例。结论 在乳腺癌的发生、发展过程中出现MSI,并可能和ER、PR的表达降低有关。
Objective To investigate the microsatellite instability(MSI) in breast carcinoma and its relationship with clinical parameters.Methods To analyze 6 microsatellite loci in 40 paired breast cancer DNA samples.PCR products were eletrophoresed and detected using silver staining.The p53,progesterone receptor(PR) and estrogen receptor(ER) status were determined by immunohistochemistry.Results Nine of the 40 breast cancers showed evidence of MSI at least one locus.There was no significant association with MSI and age,tumor size and p53.MSI was higher in the patients with ER and PR negative and lymph node involvement than those patients with ER and PR positive.Conclusion Breast cancer is associated with MSI during tumorgenesis and may be related with deregulation of ER and PR.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2005年第2期185-187,共3页
Journal of Harbin Medical University
基金
黑龙江省科学技术计划项目 (G0 0C1914 0 1)
关键词
乳腺癌
微卫星不稳定性
淋巴结
breast cancer
microsatellite instability
lymph node
