摘要
用RT-PCR方法从6个新城疫病毒(NDV)广东分离株中扩增HN基因cDNA片段,并将其克隆至pGEM-T Easy载体进行核苷酸序列测定.结果表明,6个NDV分离株HN基因片段长度均为1704bp,编码568个氨基酸;彼此间核苷酸和氨基酸同源性分别为96.0%～99.8%和98.6%～100%,与其他基因Ⅶ型毒株的氨基酸序列同源性为96.8%～98.4%;但与其他基因型毒株如D26、Ulster/67、B1、LaSota以及GB Texas的氨基酸同源性较低,为88.2%～91.0%.
The cDNA fragments of HN gene of 6 Newcastle disease viruses(NDV)from Guangdong Province were amplified by RT-PCR. The HN fragments were cloned into pGEM-T Easy vector and the recombinant plasmids were sequenced. The results showed that the HN gene fragments from the 6 NDV (isolates) consisted of 1704bp, coding for 568 amino acids. Neucleotide and amino acids sequence homology were found to be from 96.0% to 99.8% and from 98.6% to 100% respectively. Moreover, the amino acids sequence homology between the 6 isolates and genetype Ⅶstrains, categorized by restriction site and F gene sequence, also came to from 96.8% to 98.4%, but the homology to other genetypes of NDV strains such as D26,Ulster/67,B1,LaSotaand GB Texas were only from 88.2% to 91.0%.
出处
《中国兽医科技》
CAS
CSCD
北大核心
2005年第3期194-198,共5页
Chinese Journal of Veterinary Science and Technology
基金
国家高技术研究发展计划(863)项目(2002AA245051)
