摘要
取3个不侵染马铃薯的番茄晚疫病菌菌株和3个不侵染番茄的马铃薯晚疫病菌菌株,以及2个可交叉侵染马铃薯和番茄的菌株,提取DNA全基因组后,用引物ITS1和ITS4进行扩增,得到约850bp左右的扩增产物并进行测序,比较同源性,结果显示这些菌株的全基因组DNA序列与Genebank上登录的3个序列AF228084,AF228083,AY269995 1的同源性均为100%。3个番茄菌株之间的同源性大小为98 22%,3个马铃薯菌株之间的为99 52%,较番茄菌株的同源性高。不可交叉的两类菌株间的同源性为98 28%,可交叉侵染的番茄菌株和马铃薯菌株之间的同源性为99 17%,比不可交叉侵染的菌株间的同源性高,说明相似的序列使得生物学特性也很相似,反之,同源性有差异的,在生物特性上也表现出一定差异。
3 isolates of P.infestans from tomato that couldn't infect ptato,3 isolates from potato that couldn't infect tomato and 2 isolates of P.infestans that could infect both tomato and potato was amplified using polymerase chair reaction(PCR) and the universal primers internal transcribed spacer ITS 1 and ITS4.PCR products wee an approximately 850 base bair and checked the sequences. The sequences of ribosomal DNA-ITS of 8 isolates were compared with 3 sequence logging in genebank and their homegenies were 100%. The homologous character of 3 isolates from tomato were 98.22% and 3 isolates from potato were 99.52%. The homogenies of 2 isolates that couldn't cross infect potato and tomato were 98.28%. The homogenies of 2 isolates that could infect tomato and potato were 99.17%,it was higher than isolates that couldn't cross infect. The resembling sequences leaded to the resemble biological characters. On the contrary, the different sequences leaded to different biological characters.
出处
《云南农业大学学报》
CAS
CSCD
2005年第2期188-192,共5页
Journal of Yunnan Agricultural University
基金
云南省自然科学基金项目(97C023M)
云南省科技攻关项目(2002NG09)
关键词
晚疫病菌
交叉侵染
ITS区段序列
Phytophthora infestans
crossed infection
ITS region sequence
