摘要
目的:研究中国广东人降钙素基因相关肽(calcitoningene-relatedpeptide,CGRP)基因多态性与成人重度牙周炎易感性的关系。方法:收集40例成人重度牙周炎患者和46例健康对照组的颊黏膜拭子,抽提DNA,通过PCR扩增出CGRP基因片段。结果:基因测序法证明该扩增序列来自CGRP基因(参照Gen bank收录的X15943CGRP基因序列)。进一步检测来自重度牙周炎患者的扩增CGRP基因序列,发现在检测的CGRP+5247位点,发生了A-C单核苷酸基因多态性(SNP)。结论:CGRP+5247位点发现等位基因杂合子多态性现象的存在;CGRP+5247位点单核苷酸多态性,可能成为中国广东人牙周炎的易感因素之一。
AIM:To investigate the relationships between the CGRP gene polymorphisms and the susceptibility to severe adult periodontitis in Canton region.METHODS:Buccal swabs were collected from adult patients with severe adult periodontitis and healthy adult controls.DNA was extracted from each sample using Chelex-100 method.CGRP related gene fragments were amplified by the PCR techniques.The amplified gene fragments were sequenced directly.Sequencing data were analyzed with bioinformatics to detect the CGRP related genotype polymorphisms.RESULTS:It was demonstrated that the amplified sequences have great homology with that of the CGRP gene,as compared with the CGRP sequence of GenBank accession number:X15943.Further analysis of the sequences from the periodontitis patient could revealed that there were detectable SNP polymorphism (CGRP+5247 A>C) in the CGRP+5247 site.CONCLUSION:There is a SNP existed in the CGRP+5247 allele,which may be one of the makers of susceptibility to severe periodontitis patients in Canton region.
出处
《牙体牙髓牙周病学杂志》
CAS
2005年第4期197-201,共5页
Chinese Journal of Conservative Dentistry
基金
上海市自然科学基金资助课题 (02ZB14001)
关键词
降钙素基因相关肽
基因多态性
重度牙周炎
calcitonin gene-related peptide (CGRP)
genes polymorphisms
periodontitis
