摘要
目的:本研究拟探讨丹参酮ⅡA对肺癌细胞株NCI- H460的增殖抑制作用及其作用机理。方法:应用MTT比色法检测不同浓度丹参酮ⅡA对NCI- H460细胞的增殖抑制,应用流式细胞仪观察丹参酮ⅡA对NCI- H460细胞周期的影响,应用RT- PCR法检测用药后bcl 2和C myc基因mRNA表达水平。结果:丹参酮ⅡA对NCI H460细胞增殖抑制作用成剂量依赖性。细胞周期分析显示:用0 5μg/ml丹参酮ⅡA作用NCI H460细胞24、48、72h后,凋亡细胞分别占细胞总数1 .2%、3 .4%、7. 7%;用1 0μg/ml丹参酮ⅡA作用24、48、72h,凋亡细胞分别占细胞总数2 .6%、5. 9%、13 .4%;用2 0μg/ml丹参酮ⅡA作用24、48、72h,凋亡细胞分别占细胞总数4 .1%、8 .7%、37. 6%,说明丹参酮ⅡA能促使NCI H460细胞发生凋亡,且此作用呈剂量依赖性。用药48h后bcl- 2和C -myc基因mRNA表达明显降低。结论:丹参酮ⅡA对NCI H460细胞的增殖具有明显的抑制作用及诱导凋亡的作用。关键词 丹参酮ⅡA;NCI H460细胞株;细胞周期;
Objective: To investigate the inhibition of tanshinone ⅡA in NCI-H460 cell line and its mechanism.Methods:MTT was used to measure the levels of the proliferation of NCI-H460 cultured with tanshinone ⅡA in different concentrations.The effects of tanshinone ⅡA on cell cycle of NCI-H460 were observed by FCM. After treated with tanshinone ⅡA for 48h, the level of mRNA of bcl-2 and C-myc in NCI-H460 was tested by RT-PCR method.Result:The proliferation of NCI-H460 was obviously inhibited by tanshinone ⅡA in a dose dependent manner.The outcome of FCM showed that the apoptotic cell rate was 1.2%,3.4%,7.7% respectively,when cultured with tanshinone ⅡA at 0.5 μg/ml for 24,48,72h. The apoptotic cell rate was 2.6%,5.9%,13.4%,when cultured with tanshinone ⅡA at 1.0 μg/ml for 24,48,72h. The apoptotic cell rate was 4.1%,8.7%,37.6%,when cultured with tanshinone ⅡA at 2.0 μg/ml for 24,48,72h. The outcome of RT-PCR showed that the expression of proto-onco gene bcl-2 and C-myc was notably decrease,after cultured with tanshinone ⅡA for 48h.Conclusion:Tanshinone ⅡA can inhibit the proliferation of NCI-H460 and induce the apoptosis of the cell.
出处
《中药材》
CAS
CSCD
北大核心
2005年第4期301-304,共4页
Journal of Chinese Medicinal Materials
