摘要
目的评价重组华支睾吸虫26kDaGST蛋白(rCs26GST)在华支睾吸虫病血清学诊断上的价值。方法使用rCs26GST抗原,以酶联免疫吸附法(ELISA)和免疫增强化学发光法(immuno-enhancedchemiluminescence,immuno-ECL)检测华支睾吸虫病者、其它寄生虫感染者及健康人血清特异性IgG和IgE抗体,分析rCs26GST抗原用于检测的敏感性和特异性。结果使用rCs26GST抗原,ELISA法检测了30份华支睾吸虫感染者血清、肺吸虫病人血清15份、日本血吸虫病人血清20份、无寄生虫感染的健康人血清40份,华支睾吸虫感染者血清IgG的阳性检出率为73.3%(22/30),健康人、肺吸虫病和日本血吸虫病人的阴性率分别为92.5%(37/40)、80.0%(12/15)、85.0%(17/20)。Immuno-ECL法检测华支睾吸虫感染者血清rCs26GST-特异性IgE的阳性检出率为86.7%(26/30),肺吸虫病为26.7%(4/15),日本血吸虫病人和阴性对照组的血清未测出,此法的特异度是95.5%。结论rCs26GST蛋白用于血清特异性IgG和IgE抗体的检测具有较高的敏感性和特异性,可用于华支睾吸虫病的血清学诊断,组合成“鸡尾酒”式的复合诊断抗原之一。
Objective To evaluate the application value of recombinant 26kDa Glutathione S-tr ansferase protein of C.sinensis(rCs26GST)for the serodiagnosis of human clonorchiasis.Methods rCs26GST was used to detect clonorch iasis by ELISA and Immuno-ECL.Serum sampl es from other parasitic infections a nd healthy individuals were used as c ontrol.The sensitivity and specificity of rCs26GST antigen used for the detection w ere analyzed.Results30cases of C.sinensis infected sera were analyzed with a positive detection rate of the IgG antibody of 73.3%(22/30)for the rCs26GST antigen;40cases of healthy individual sera,15cases of paragonimiasis sera and 20cases of schistosomiasis sera were also analyzed,the negative rates were 92.5%(37/40),80.0%(12/15)and 85.0%(17/20)respectively.rCs26GST-specific I gE was detected,in 86.67%(26/30)of the sera from clonorchiasis patie nts,in 26.7%(4/15)of the sera of paragonimiasis patients,but was not detected in the sera of schistosomiasis patients an d the uninfected humans used as contr ols.The specificity of rCs26GST for IgE was 95.5%by the immuno-ECL.Conclusion rCs26GST protein has high sensitivity and specificity for the detection of serum specific IgG and IgE antibody so th at it can be used as a component of an an tigen cocktail for the serodiagnosis of human clonorchiasis.
出处
《热带医学杂志》
CAS
2005年第2期197-199,共3页
Journal of Tropical Medicine
