摘要
本研究采用组织培养、高效液相色谱等方法，确定了羊草的休眠类型为抑制物引起的生理性休眠，主要抑制部位是胚乳，关键抑制物之一是脱落酸（ＡＢＡ）；羊草种子的休眠期为４年左右，５至６年达到自然发芽率高峰，此后发芽率开始下降；外源植物激素ＣＡＳ和６─ＢＡ对解除羊草种子的休眠具有促进作用。贮存１年的深度休眠的羊草种子，用低温流水浸泡法处理后能使自然发芽率（土培法，３５．０％）提高到８８．５％（土培法）．不同发芽床上进行的发芽率比较试验表明，土培法和沙培法明显优于纸培法，以土培法的发芽率为最高。
o solve the problem of poor germination in Leymus chinense seeds in cultivation,the re-search work was priorly focused on dormancy.By introducing the methods of tissue cul-ture and high-performance liquid chromatography,etc,it was confirmed that the dor-mant state was imposed by inhibitors.The inhibitory site was endosperm,and ABAwas the main inhibitor.It was also determined that the duration of dormancy for L。chinense seeds was about 4 years. The peak of natural germination appeared in the 5thor 6th year after harvest,and then the persentage of germination tended to decrease.The exogenous hormones(GA_3,6─BA),showed positive effects tor breaking dor-mancy. The percentage of germinaton in L.chinense seeds treated by cold flowing wa-ter changed markedly.Germination percentage of deep dormant seeds preserved for lyear increased from 35.0%(control)to 88.5%in soil culture. The relationship be-tween the seed preservation period,the duration of cold flowing water treatment andthe percentage of germination in seeds,and the possible mechanism of cold flowing wa-ter treatment for improving germination rate were discussed.The comparison of naturalgermination rates from 3 different germination beds showed that the percentage of ger-mination was the highest in soil culture and higher in sand culture than in paper culture.
出处
《中国草地》
CSCD
1994年第6期1-6,共6页
Grassland of China
基金
国家自然科学基金
关键词
羊草
种子
休眠生理
发芽率
Leymus chinense seeds, Dormant physiology,Germination rate
