摘要
目的 研究乳腺癌P5 3、CA15 3表达与肿瘤分化、浸润转移的关系及其临床意义。方法 分别应用免疫组化法(IHC)及血清放射免疫分析法(IRMA) ,检测P5 3、CA15 3在6 3例乳腺癌及2 5例乳腺良性病变中的表达情况。结果 在6 3例乳腺癌中P5 3和CA15 3阳性率分别为33 3%和4 9 .2 % ,CA15 3血清含量为38. 3±2. 9 3U/ml,均明显高于良性对照组(P <0 . 0 1) ;乳腺癌P5 3阳性率与组织学分级、肿瘤大小、临床分期呈正相关(P <0 .0 5 ) ;乳腺癌CA15 3血清含量及阳性率与组织学分级、肿瘤大小、腋窝淋巴结转移呈正相关(P <0 .0 5 ) ,CA15 3含量还与临床分期呈正相关(P <0 . 0 5 ) ;乳腺癌P5 3阳性表达与CA15 3血清含量呈正相关(P <0 0 5 )。结论 乳腺癌P5 3、CA15 3的表达与肿瘤发生发展、生长分化或转移密切相关;检测这些指标,有助于临床对乳腺癌恶性程度及预后的判断或早期诊断、病情监测。
Objective To investigate the expression of P53 and CA153 in patients with breast carcinoma, to seek their relationship with tumor differentiation, invasion and metastasis, and to study their clinic significance. Methods Immunohistochemistry(IHC) method and serum immunoradiometric assay(IRMA) was used to detect the expression of P53 and CA153 respectively, in 63 cases of breast carcinoma and 25 cases of benign breast disease. Results In 63 cases of breast carcinoma the positive rates of P53, CA153 were 33.3% and 49.2%, respectively, the concentration of serum CA153 was 38.3±29.3 U/ml, which were all significantly higher than that in benign control group( P <0.01); the positive expression of P53 was related to histopathologic grade, tumor size, clinical stage( P <0.05); the serum concentration and the positive rates of CA153 in breast carcinoma was related to histopathologic grade, tumor size and axillary lymph node metastasis( P <0.05), and the serum concentration of CA153 was also related to clinical stage( P <0.05); the positive expression of P53 was related to the serum concentration of CA153( P <0.05). Conclusion The expression of P53 and CA153 closely correlates with the carcinognesis, development, growth, differentiation or metastasis of breast carcinoma; detecting these indexes can be helpful in assessment of the malignancy grade and prognosis, or the early diagnosis and surveillance of breast carcinoma.
出处
《中国实验诊断学》
2005年第2期221-224,共4页
Chinese Journal of Laboratory Diagnosis
