摘要
选取产蛋性能具有明显差异的 4个鸡品种(莱航鸡、阳山鸡、丝羽乌骨鸡和隐性白洛克鸡 )构建品种DNA池,采用测序的方法研究鸡催乳素基因 5′侧翼调控区远端序列 (1 028bp)的多态性,快速筛查到 8个可能与产蛋性能相关的SNPs(C 2402T、T 2192C、C 2161G、C 2134G、C 2062G、G 2040A、A 1944G和C 1884A)。进一步利用测序图中SNP等位基因峰高的比值估算各鸡品种等位基因的频率,其中C 2402T、C 2161G、C 1884A和C 2062G、G 2040A位点等位基因频率的估算结果分别被PCR RFLP、PCR SSCP所验证,说明测序峰高比值估算等位基因频率的方法具有一定的可行性。
Four breeds of chickens (White Leghorn,Yangshan,Taihe Silkies,White Recessive Rocks) with different egg production were applied to screen potential single nucleotide polymorphisms (SNPs) related to reproduction trait in distal part region of prolactin gene based on DNA pooling and sequencing.Eight SNPs (C-2402T,T-2192C,C-2161G,C-2134G,C-2062G,G-2040A,A-1944G and C-1884A) were found successfully in the fragment of 1 028 bp at the distal part of 5′flanking region.Moreover,a simple and rapid method to estimate allelic frequencies of SNPs in each breed by calculating the ratio of allele peak heights was introduced here.The accuracies of estimation for the allelic frequencies of C-2402T,C-2161G,C-1884A,C-2062G and G-2040A were verified by comparing with the results of PCR-RFLP or PCR-SSCP.
基金
国家重点基础研究发展规划项目(编号:G2000016102 )资助~~
