摘要
在水稻基因组序列中发现类似MYC序列的同源序列,并设计引物成功克隆了一个水稻OsMYC基因(GenBank登录号:AY398581 ),并对其进行了分子结构分析。OsMYC基因具有典型的DNA结合结构域: 碱性区域 /螺旋 环 螺旋(bHLH)基序,与其他MYC类似基因的蛋白序列比对结果表明,OsMYC基因与AtMYC2、MYC7E和PG1等氨基酸序列一致性分别是 78%、48%、46%,而在bHLH区的一致性分别为 95%、84%、77%,N端保守区的一致性分别为 81%、54%、52%;位于bHLH区域内的核定位信号区则完全一致;系统进化树分析结果表明,该基因与AtMYC2、MYC7E和PG1等位于同一亚类。此外,该基因主要在营养器官中表达,茎秆中表达最强,根和叶片中较弱,并且能被外源ABA或Fe3 +所诱导,这与AtMYC2、MYC7E基因的表达模式基本相同。该基因是水稻MYC转录因子家族的一个新成员。
A MYC(Myelocytomatosis) transcription factor gene,OsMYC,was cloned in rice (Oryza sativa L.).The putative protein of the OsMYC gene has a typical DNA binding domain: basic/helix-loop-helix (bHLH) motif at the C-terminus.Its nucleocide sequnce has 78%,48%,46% identity with that of the AtMYC2,MYC7E and PG1 genes,respectively.The identity of deduced amino acid between OsMYC and AtMYC2,MYG7E and PG1 is 95%,84%,and 77% in the bHLH domain,and that is 81%,54% and 52% in the N-terminus conserved region.The identity is 100% in the nuclear localization signal between them.Phylogenetic comparisons revealed that the OsMYC protein was related to the Arabidopsis AtMYC2,Zea MYC7E and Phaseolus vulgaris PG1.OsMYC gene was expressed at high level in stem,but low level in leaf and root and can be induced by ABA and Fe^(3+),which is similar to the expression pattern of MYC7E and RAP1 genes.Thus,this gene is a new member of the rice MYC family.
基金
国家"973"计划(编号: 2003CB114300 )~~
关键词
水稻
MYC转录因子
克隆
rice (Oryza sativa L.)
MYC transcription factor
cloning
