摘要
利用涂布平板法从小麦根系中分离出8株内生细菌,从中筛选出1株对小麦纹枯菌(Rhizoctoniacerealis)具有拮抗作用的内生菌。室内测定该菌株培养液对小麦纹枯病菌的抑制作用,结果发现,小麦纹枯病菌在培养液中生长缓慢,培养6d后菌丝量与对照相比下降了89%,同时发现病菌菌丝生长畸形,出现断裂和细胞壁瓦解。双抗标记法测定该拮抗菌在小麦根系中的定殖情况,发现该菌能够在根系中长期定殖。初步的鉴定结果表明该菌为蜡样芽孢杆菌。
8 strains of endophytic bacteria were isolated from roots of wheat.,from that one strain was acquired after screening which had antagonism on the wheat pathogenic fungi of Rhizoctonia cerealis .The inhibitory action to the fungi was assayed in the lab using the fermentable extract of antagonistic endophytic bacteria, the results showed that mycelium of Rhizoctonia cerealis growed slowly and the biomass reduced 89% compared with the control after inoculation 6 days.The hyphae ,at the same time,were deformative which broke into pieces. Colonization was studied with dual-resistant label which suggested that the endophytic bacteria strain could colonize in the root systems of wheat. Preliminary characterization indicated that the antagonistic bacteria was Bacillus cereus.
出处
《微生物学通报》
CAS
CSCD
北大核心
2005年第2期20-24,共5页
Microbiology China
基金
河南省骨干教师资助项目(No.G2002004)
关键词
内生细菌
拮抗作用
小麦纹枯菌
生物防治
Endophytic bacteria, Antagonism, Rhizoctonia cereali, Bio-control
