摘要
目的:将骨髓基质细胞(MSC)扩增后植入帕金森病(PD)鼠的纹状体内,观察其在患鼠脑内的生存、分化、迁移等变化,以期为研究和治疗PD提供新的思路和方法。方法:建立PD鼠的动物模型,提取PD鼠的骨髓,并进行培养、分离和扩增,得到大量MSC,在用BrdUrd标记后,立即进行同种同体脑移植,移植后PD鼠存活20天～3个月后处死。取PD鼠脑组织,用BrdUrd抗体进行免疫荧光检测,用胶质原纤维酸性蛋白特异抗体和神经原纤维抗体进行双重免疫组化检测,检测植入纹状体中供者细胞的存活、迁移情况和是否分化为星形胶质细胞和神经细胞。结果:植入的MSC能向脑的许多部位进行迁移,特别是向双侧的纹状体和黑质迁移。在核BrdUrd荧光染色阳性细胞的细胞质中可见到胶质原纤维酸性蛋白棕红色的阳性产物,但是未观察到神经原纤维的阳性产物。结论:在PD鼠脑的微环境中,MSC能向脑的多个部位进行迁移,并分化为星形胶质细胞。
Objective: To observe the survival, differentiation and migration of marrow stromal cells(MSC) transplanted into brain of Parkinson’s disease (PD)rats after proliferated. Methods: After establishing PD rat model,the bone marrow of PD rat was obtained, sisolated, cultured and proliferated for obtaining a great deal MSCs. These autologous MSCs marked with BrdUrd were transplant into brain of PD rat. These rats were killed after transplanted for 20 days-3 months and their brains wers obtained. Then obtained brains were stained immunofluorescencally for BrdUrd and immunohistochemically for GFAP and NF. Results: MSCs transplanted can migrate to many places of brain of PD rats, especially the double striatum and substantia nigra. Double labeling for BrdUrd and GFAP revealed that some of the MSCs within the corpus striatum differentiated into astrocytes. In contrast, none of the cells in these regions stained positive for neurofilament. Conclusion: In the microenvironment of PD brain rats MSCs can migrate to many places as normal rats and can differentiate into astrocytes.
出处
《医学研究生学报》
CAS
2005年第4期327-329,333,F004,共5页
Journal of Medical Postgraduates
基金
江苏省科技厅应用基础基金资助项目(BJ2000003)
关键词
骨髓基质细胞
帕金森病
大鼠
Bone marrow stromal cells
Parkinson’s disease
Rat
