摘要
目的:探讨不同牙科合金对局部龈沟液中肿瘤坏死因子α以及白细胞介素6表达的影响。方法:实验于2003-01/08在第四军医大学动物中心实验室完成,在实验犬的口腔中制作全冠(钴铬合金、镍铬合金、金合金、纯钛),在修复后第1个月和第6个月,用WhatmanI号滤纸采集龈沟液样本。用酶联免疫吸附法测定龈沟液中白细胞介素6和肿瘤坏死因子α的含量。结果:4种全冠对肿瘤坏死因子α的影响,在修复后第1,6个月时钴铬合金、镍铬合金、纯钛与对照组相比有显著增加(P<0.05);而金合金和对照组相比差异无显著性意义(P>0.05);仅镍铬合金对肿瘤坏死因子α分泌量的影响在时间上差异有显著性意义(P<0.05);钴铬合金、镍铬合金之间差异无显著性意义(P>0.05),与金合金比较显著增加(P<0.05);纯钛材料与其余4种材料差异均无显著性意义(P>0.05)。而4种全冠都没有引起白细胞介素6分泌量的显著增加(P>0.05)。结论:不同的牙科合金材料对龈沟液中某些细胞因子的含量有影响,从而影响患者的术后康复。
AIM:The aim of this study is to further investigate the effect of four kinds o f dental alloys on cytokines,tumor necrosis factor α(TNF α) and interleukin 6 (IL 6) in gingival crevicular fluid(GCF). METHODS:The experiment was conducted in the central laboratory for animals,Fou rth Military Medical University of Chinese PLA from January to August 2003.Four kinds crowns(cobalt chrome,nickel chrome,pure titanium and gold) were fabricat ed in the dog oral cavity .On the 1st month and 6th month after cementation,GCF was obtained by Whatman I filter paper strips,and the level of IL 6 and TNF α in GCF was detected by enzyme linked immunoadsorbent assay(ELISA) method. RESULTS:After the four kinds crowns cemented,on the 1st and 6th month,cobalt chrome,nickel chrome and pure titanium led to more significant influence on TNF αlevel than the controls(P< 0.05),except the gold(P >0.05);Only nickel chrom e had an obvious difference in TNF αsecretion between 1st and 6th month(P< 0.0 5);Cobalt chrome and nickel chrome were insignificant in leading to secretion of TNF α(P >0.05),but either was significant as compared with gold and porcela in crowns(P< 0.05);Between pure titanium and the others there was insignificant difference(P >0.05);The four kinds of crowns all had no significant effect on se cretion of IL 6(P >0.05). CONLUSION:Different dental alloys may have different effect on production of s ome cytokines in GCF,thus influencing the postoperative rehabilitation.
出处
《中国临床康复》
CSCD
北大核心
2005年第14期48-49,共2页
Chinese Journal of Clinical Rehabilitation
