摘要
目的:初步探讨特应性皮炎(AD)患者外周血单个核细胞(PBMC)Th1、Th2的状态及超抗原的致病机制。方法:分别用金黄色葡萄球菌(金葡菌)肠毒素B(SEB)和植物凝血素(PHA)刺激体外培养的AD患者及健康对照组PBMC,双抗体夹心ELISA法检测PBMC产生的白介素(IL)-4、干扰素(IFN)-γ的含量。结果:PHA刺激后,AD患者PBMC产生的IFN-γ明显低于健康对照组(P<0.01),而两组间IL-4水平差异无显著性(P>0.05);SEB刺激后,AD患者PBMC产生的IL-4水平比健康对照组明显增高(P<0.01),IFN-γ水平明显降低(P<0.01)。结论:AD患者PBMC经SEB刺激后,Th2、Th1细胞亚群失衡,表现为Th2细胞亚群呈优势表达。
Objective: To explore the etiology and pathogenesis of atopic dermatitis and the correlation between atopic dermatitis (AD) and the immune response. Methods: By using ELISA we detected IL-4 and IFN-γ levels secreted by the peripheral blood mononuclear cells(PBMC) which had been stimulated by staphylococcal enterotoxin B (SEB) and PHA respectively. Results: After peripheral lymphocytes were stimulated by PHA, IFN-γ level was markedly decreased in the patients (P < 0.05), but there was no significant difference in IL-4 secretion between the patients and the controls (P > 0.05). IL-4 was significantly higher (P < 0.01) and IFN-γ was strikingly lower (P < 0.01) after PBMC were treated with SEB in patients compared with the controls. Conclusions: When PBMC are stimulated by SEB, the subgroups of T helpers in patients with AD might have a deviation which would lead to priority of Th2 cytokine secretion.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2005年第5期287-288,共2页
Journal of Clinical Dermatology
关键词
皮炎
特应性
金黄色葡萄球菌
超抗原
白介素-4
γ-干扰素
atopic dermatitis
staphylococcal aureus superantigen
IL-4
IFN-γ [J Clin Dermatol, 2005, 34(5):287-288]
