摘要
小分子热激蛋白与植物耐寒性提高有相关性,但是没有直接的实验证据能证明小分子热激蛋白的存在增加植物抗寒性。我们克隆了番茄叶绿体(定位)小分子热激蛋白cDNA,并将35SCaMV启动子驱动的番茄叶绿体小分子热激蛋白cDNA植物表达构架导入番茄,测定转基因番茄和未转基因番茄的抗寒性水平。低温处理后,转基因番茄的冷害症状轻于未转基因的番茄;转基因番茄细胞电解质外渗较少、花青素和MDA累积量较低;净光合速率和叶绿体含量高于对照。这些实验结果说明叶绿体小分子热激蛋白的过量表达提高了植物抗寒性。
There are many reports about the correla-tion between small molecular heat-shock protein(sHSP) and the acquirement of chilling tolerance, butno direct evidence that sHSP confers enhanced chill-ing tolerance to plant has been reported. A DNAconstruct, including tomato chloroplast-localized smallmolecular heat-shock protein (CPsHSP) cDNA underthe control of cauliflower mosaic virus 35S (35SCaMV)promoter, was introduced into the genome of tomatoplants. The chilling tolerance of the transgenic tomatolines and the non-transgenic tomato was evaluated.After exposure to chilling stress, the transgenic plantsexhibited lighter chilling-injured symptoms, sufferedless electrolyte leakage and less destruction ofchlorophyll, accumulated less anthocyanins and lessMDA and kept higher value of net photosynthetic rate,than non-transgenic plant. All results indicated consis-tently that transgenic tomato plants had stronger chill-ing tolerance. These characters are ascribed to con-stitutive expression of cpshsp and lead to the conclu-sion that HSP can enhance chilling tolerance in plant.
出处
《植物生理与分子生物学学报》
CAS
CSCD
北大核心
2005年第2期167-174,共8页
Journal Of Plant Physiology and Molecular Biology
基金
国家自然科学基金(No. 30270132)
山东省青年科学家基金资助~~
关键词
叶绿体
叶绿体小分子热激蛋白
耐寒性
转基因番茄
热激蛋白
chloroplast
chloroplast-localized small molecularheat-shock protein
chilling tolerance
transgenic tomato
HSP
