摘要
目的:测定兔口服阿魏酸及口服阿魏酸加生化汤后阿魏酸的血药浓度,并进行药动学比较。方法:采用高效液相色谱法(HPLC)以甲醇水(0.3%冰醋酸)(40∶60)为流动相,色谱柱为Zorbax SBC18(150 mm×4.6 mm,5μm),紫外检测波长323 nm。结果:两组的血药-时曲线均表现为单峰现象,属开放性一室模型。兔口服阿魏酸及兔口服阿魏酸加生化汤后的主要药动学参数如下:Ka(min-1)=0.2061和0.3523,Ke(min-1 )= 1.013×10-2 和5.440×10-3,t1/2Ka (min)= 3.363和1.968,t1/2Ke (min)= 68.41和127.4,Cmax(μg·L-1)=63.41和112.7,AUC(μg·L-1·min)=7312 和2.212×104,CL/F(s)(mL·min-1 )=1.360×10-4 和3.420×10-4,V/F(c)(μg·L-1)=2.493×10-2和3.374×10-2。结论:生化汤对阿魏酸的体内吸收、分布、代谢均产生影响。
OBJECTIVE To determine the concentration of ferulic acid in plasma of rabbits after given either ferulic acid or ferulic acid and Shenghua Tang , then to compare them on pharmacokinetics.METHODS HPLC was used with Zorbax SBC_ 18 column( 150 mm× 4.6 mm,5μm),methanol- water (containing 0.3% acetic acid )(40∶60) as the mobile phase and UV detection at 323 nm.RESULTS Both groups gave serum ferulic acid concentration-time curves with one peaks showing their open one-compartment model nature ,the main pharmacokinetic parameters were:Ka(min -1)= 0.2061 and 0.3523,Ke(min -1)= 1.013×10 -2 and 5.440×10 -3,t_ 1/2Ka(min)= 3.363 and 1.968,t_ 1/2Ke(min)= 68.41 and 127.4,C_ max(μg·L -1) = 63.41 and 112.7,AUC (μg·L -1·min)=7312 and 2.212×10 4,CL/F(s)(mL·min -1) = 1.360×10 -4 and 3.420×10 -4,V/F(c)(μg·L -1)= 2.493×10 -2 and 3.374×10 -2.CONCLUSION The above results indicated that Shenghua Tang could influence the absorption, distribution and elimination of ferulic acid in rabbits.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2005年第3期213-215,共3页
Chinese Journal of Hospital Pharmacy
基金
湖北省科技攻关项目(编号:2003AA302B02 2004AA302B09)
