摘要
将人酸性纤维母细胞生长因子’（ａＦＧＦ’）ｃＤＮＡ的ＰＣＲ产物以ＴＡ连接方式克隆入ｐＣＲ￣（ＴＭ）ＩＩ质粒，然后采用Ｔ７和Ｓｐ６启动子特异性引物对克隆的片段以双脱氧未端终止法进行双链ＤＮＡ测序。
Human
acidic fibroblast growth factor’cDNA amplified in PCR was cloned
into pCR￣(TM) IIplasmid by TA Iigation and sequenced directly by
using dideoxynucleoside chain termination re-action primed by T7 and
Sp6 primers. The results demonstrated that the technique was a
rapidand relitable method for cloning and sequening PCR products.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1994年第1期5-7,共3页
Chinese Journal of Immunology
基金
香港郑氏家族资助
关键词
DNA
聚合酶链反应
克隆
TA
DNA polymerase chain reaction cloning DNA sequencing
