摘要
通过移植并改进ＥＬＩＳＡ技术，用来检测中性粒细胞呼吸爆发时释放的Ｈ＿２Ｏ＿２量，发现ＥＨＦＶ触发中性粒细胞呼吸爆发能力明显强于正常组及ＣｏｎＡ刺激组（Ｐ＜０．０１），抗ＥＨＦＶ抗体可明显减弱其活病毒刺激中性粒细胞释放Ｈ＿２Ｏ＿２的能力（Ｐ＜０．０５）。经改进的该方法检测Ｈ＿２Ｏ＿２量灵敏度高，快速简便。
e found that neutrophil granulocytes
could release more amount of H_2O_2 being stimulatedby EHFV than by
Concanavarin A, It had been proved that H_2O_2 could injure the
endothelia andexacerbate the permeability of blood vessel,the
antibody against EHFV couldn′t enchance theEHFV exciting neutrophil
granulocytes through FcR receptor,conversely,reduce its effect
onneutrophll granulocyres,therefore, relieving the respiratory burst
might be another function ofantibody. We improved the ELISA to
quantify the amount of H_2O_2 which was sensitive,rapidlyand simple.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1994年第4期233-234,共2页
Chinese Journal of Immunology
关键词
出血热病毒
中性粒细胞
呼吸爆发
Epidemic hemorragic fever virus(EHFV)Neutophil
granuloytes(NGC)Respiratory ftirst
