摘要
用体细胞核移植的方法生产的克隆猪诞生至今已近5年,虽然同胚胎细胞核移植相比,体细胞核移植技术难度大,成功率低,但近年来用体细胞核移植技术克隆猪有了更深入的研究,在体细胞核移植基本机理和关键技术上取得了一定的进展。综述了用核移植的方法生产体细胞克隆猪的技术关键和难点及应用情况,并对提高猪体细胞核移植效率的策略进行了分析。
It has been almost five years since the first report of live pig produced by somatic cell nuclear transfer (SCNT) technology. Though it is more difficult, lower efficiency, compared to embryonic cell nuclear (ECNT) technology, the cloning pigs with SCNT technology has been further studied. Some progress has taken in the fields of mechanism and approaches. In this paper, we mainly focus on the key technology and its difficulties of SCNT in cloning pigs, including its applications and approach of increasing the efficiency of SCNT.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第5期506-510,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家高技术发展计划"863"重大专项(2002AA206621)
