摘要
应用颗粒细胞制备单层细胞时，将其浓度分为８组：６４×１０６、３２×１０６、１６×１０６、８×１０６、４×１０６、２×１０６、０．５×１０６、０个／ｍＬ，观察了不同浓度颗粒细胞对体外受精早期胚胎体外发育的影响。试验结果表明，制备单层细胞的适宜颗粒细胞浓度为（０．５～３２）×１０６个／ｍＬ，其中１６×１０６个／ｍＬ浓度组的囊胚率为４２．３±８．０％，明显高于对照组（２２．７±８．３％）及６４×１０６个／ｍＬ浓度组（２５．２±１２．Ｏ％）（Ｐ＜０．０１）。就发育速度和胚胎质量而言，该组胚胎的发育较快且质量较好，而且标准差反映批与批之间结果较为稳定，是理想的做单层浓度。
The objective of this experiment was to find out the optimum
concentra-tion of granulosa cell in preparing granulosa cell monolayers(GCM ) for the culture of
early bovine embrvos derived from in vitro maturation (IVM)and in vitro fertilization (IVF). Eight
treatments were arranged according to the concentrations of granulosa cell in preparing
GCM:64、32、16、8、4、2、0.5、0×106cell/mL, The result demonstrated that:(1) GCM was
necessary in IVC, the control had the lowest blastocysts(BL)(22.7± 8.3%)and hatched-BL(28. 4
±20.5%)rates which were significantly different from the other treatments(P<0. 01);(2)The
optimum GCM concentration was 0.5～32 × 106 cell/mL, there were no significant differences
among these concentrations in BL and hatched-BL rates (P<0. 05);(3)16 × 106 cell/mL was the
best GCM concentration for obtaining more BL, especially at day 7. Embryos in this group
developed fast and in good shape ,and results from batch to batch were rather stable。
出处
《中国兽医学报》
CAS
CSCD
1994年第2期185-187,共3页
Chinese Journal of Veterinary Science
