摘要
目的 探讨小儿细支气管炎的病毒种类及其与IL-4的关系。方法 免疫荧光法测定细支气管炎患儿鼻咽分泌物中病毒抗原, 从阳性者中随机抽取80例患儿分为呼吸道合胞病毒组、腺病毒组、流感病毒组和副流感病毒组, 每组20例, ELISA方法测定各组患儿治疗前、治愈时和治愈3个月时的血清IL-4水平。结果 治疗前, 各病毒组患儿血清IL-4水平均高于对照组健康儿(P<0. 01); 治愈时, 各组受检儿血清IL-4水平间差别无显著性意义(P>0 .05); 治愈3个月时, 呼吸道合胞病毒组患儿血清IL-4水平〔(72 8±21. 3) ng/L〕较对照儿的〔(51 .7±16. 2) ng/L〕患儿高(P<0. 01), 也较腺病毒组〔(59 .1±19. 4) ng/L〕、流感病毒组〔(57. 5±19. 5) ng/L〕及副流感病毒组〔(56 .3±18 .5) ng/L〕患儿高(P均<0 .05)。结论 呼吸道合胞病毒感染的细支气管炎治愈3个月后患儿血清IL-4水平再次升高, 说明呼吸道合胞病毒感染可能导致Th /Th失衡, 从而诱导哮喘发病。
Objective To evaluate the possible relati onship between virus types and the level of IL-4 after bronchiolitis.M ethods First,multiple respiratory viral antigens in nasopharyngeal se cretions from these infants with bronchiolitis were detected with immunofluoresc ence assay.Then,eighty cases with a history of bronchiolitis were divided into f our groups(respiratory syncytial virus group,adenovirus group,influenza virus gr oup and parainfluenza virus group) at random according to the virus they had inf ected.Finally,the level of IL-4 in serum of these cases was determined by ELISA before treatment,during recovery and three months later after recovery. Results Before treatment,the levels of IL-4 from these virus groups were significantly higher than that from control group(P<0.01),during re covery,the levels of IL-4 from all groups showed no significantly difference(P >0.05).Three months later after recovery,the level of IL-4 from respiratory syncytial virus group 〔(72.8±21.3)ng/L〕 was significantly higher than tha t from the control group 〔(51.7±16.2)ng/L〕(P<0.01),and higher than that from adenovirus group 〔(59.1±19.4)ng/L〕,influenza virus groups 〔( 57.5±19.5)ng/L〕 and parainfluenza virus group 〔(56.3±18.5)ng/L〕 also (P<0.05).Conclusion The level of IL-4 increases agai n three months later after recovery in these infants with bronchiolitis infected by respiratory syncytial virus,which indicates that the respiratory syncytial v irus may modify Th 1/Th 2 imbalance,and leads to the development of asthma.
出处
《中国全科医学》
CAS
CSCD
2005年第10期828-829,共2页
Chinese General Practice
