摘要
背景:重复性经颅磁刺激的神经保护作用已有许多研究。目的:观察重复性经颅磁刺激仪刺激后,体外培养的大鼠海马神经元的形态、活力的变化,以验证对其神经元的保护作用。设计:完全随机对照动物实验。单位:一所军医大学的神经生物研究所。材料:实验于2004-04/06在解放军第四军医大学神经生物研究所完成。取新生SD大鼠的海马做神经元原代培养,将培养细胞随机分为对照组、重复性经颅磁刺激组、过氧化氢组和重复性经颅磁刺激-过氧化氢组,每组10孔。方法:取新生SD大鼠的海马做神经元原代培养,接种48h后重复性经颅磁刺激组和重复性经颅磁刺激-过氧化氢组细胞予1Hz100mT的磁刺激1000次,对照组和过氧化氢组不予磁刺激,重复性经颅磁刺激-过氧化氢组和过氧化氢组均在接种56h后于培养液中添加过氧化氢。接种72h后,倒置相差显微镜下观察重复性经颅磁刺激组和对照组海马神经元的形态并用四甲基偶氮唑盐方法检测各组海马神经元的活力。主要观察指标:重复性经颅磁刺激组和对照组海马神经元的形态及各组海马神经元活力。结果:细胞接种72h后,重复性经颅磁刺激组和对照组细胞均呈团簇样聚和,折光性良好;胞体饱满,呈圆形、梭形、锥形,周围有光晕;细胞突起明显,多为20～30μm,形成较密集的神经网络,两组细胞形态无明显差异。
BACKGROUND:Much has been studied on the neuroprotective effect of repetitive transcranial magnetic stimulation.OBJECTIVE:To observe the effects of repetitive transcranial magnetic stimulation on the morphology and vitality of rat hippocampus neurons in vitro in order to verify its protective effect on neurons.DESIGN:A completely randomized controlled experiment with animals as subjects.SETTING:Institute of neuroscience of a military medical university of Chinese PLA.MATERIALS:The experiment was conducted at the Institute of Neuroscience,Fourth Military Medical University of Chinese PLA,from April to June 2004.Primary cultured hippocampus neurons of neonatal SD rats were used in the experiment.The cells were randomly assigned to control group,repetitive transcranial magnetic stimulation group,H2O2 group and repetitive transcranial magnetic stimulation H2O2 group,each group having 10 wells.METHODS:Hippocampus neurons of the rats were cultured by common culture method.Repetitive transcranial magnetic stimulation group and repetitive transcranial magnetic stimulation H2O2 group were treated with 1 Hz 100 mT repetitive magnetic stimulation for 1 000 times 48 hours after being seeded,whereas the control group and H2O2 group were left untreated.H2O2 group and repetitive transcranial magnetic stimulation H2O2 group were incubated with 100 μ mol/L H2O2 56 hours after being seeded.Seventy two hours after being seeded,the cellular morphology of repetitive transcranial magnetic stimulation group and control group was observed under an inverted phase contrast microscope.Cell vitality was assayed with 3 (4,5) dimethythioazol 2 yl 2,5 diphenyl tetrazoliumbromide method(MTT).MAIN OUTCOME MEASURES:The morphology and viability of the neurons in repetitive transcranial magnetic stimulation group and control group.RESULTS:Seventy two hours after being seeded,the cellular morphology of repetitive transcranial magnetic stimulation group and control group was observed under an inverted phase contrast microscope.Cells clustered and had good refractive power.The cell body was satiated and took round,fusiform or conical shape.Processes were obvious(mostly 20- 30 μ m) and formed intensive neural network.The two groups did not differ significantly in morphological alteration.MTT metabolic rate:It was higher in repetitive transcranial magnetic stimulation group[(104.43± 2.76) % ] than in control group[(100.00± 3.20) % ](F=1.344,P< 0.05);it was higher in repetitive transcranial magnetic stimulation H2O2 group[(52.61± 2.64) % ] than in H2O2 group[(46.28± 2.04) % ](F=1.675,PCONCLUSION:After repetitive transcranial magnetic stimulation,the morphology of hippocampus neurons cultured in vitro does not change obviously,but cell vitality and ability of anti oxidation are increased remarkably,which does not cause obvious harm to the cultured cells and may have some neuroprotective effects.
出处
《中国临床康复》
CSCD
北大核心
2005年第17期208-209,F003,共3页
Chinese Journal of Clinical Rehabilitation
