摘要
目的 探讨艾滋病病毒(HIV)和丙型肝炎病毒(HCV)重迭感染者两病毒载量间及其与T淋巴细胞计数的相关性。方法 采用流式细胞技术和荧光定量PCR技术,对1 5例HIV和HCV重迭感染者进行了CD+ 3 、CD+ 4 、CD+ 8淋巴细胞计数和病毒核酸载量测定,并选用多种数学模型进行相关性分析。结果 单因素相关回归分析显示:HIV病毒载量与CD+ 3 和CD+ 4 细胞计数分别呈现良好的负相关关系(r=- 0 . 6 0 1 3,P =0 .0 1 77;r =- 0. 882 8,P =0 . 0 0 0 0 ) ,HCV病毒载量与CD+ 3 和CD+ 4 细胞计数分别呈现良好的正相关关系(r =0 . 5 931 ,P =0 . 0 1 98;r =0 . 86 2 7,P =0 .0 0 0 0 ) ,HIV和HCV病毒载量间存在统计学负相关关系(r =- 0 . 895 4 ,P =0 . 0 0 0 0 )。多因素线性相关回归分析表明:CD+ 3 和CD+ 4 细胞计数及HCV病毒载量与HIV病毒载量间均呈现良好的统计学负相关关系(r=- 0 . 6 0 5 1 ,P =0 . 0 1 6 9;r=- 0 . 882 8,P =0 . 0 0 0 0 ;r =- 0 . 895 4 ,P =0. 0 0 0 0 )。逐步回归分析显示:CD+ 4 细胞计数和CD+ 4 /CD+ 8比值及HCV病毒载量与HIV病毒载量间分别存在统计学负相关关系。结论 HIV/HCV重迭感染时,两病毒间表现出竞争性抑制或干扰现象,导致CD+ 4 细胞计数多样化改变并呈现出下降趋势。
Objective To sutdy correlation between viral loads and T lymphocyte counts in HIV and HCV super-infected patients.Methods Techniques of fluorescent quantitative PCR and Flow Cytometry were used to detect viral loads of HIV and HCV and CD+_3?CD+_4?CD+_8 cell counts in 15 HIV and HCV super-infected patients,and the results were analyzed by multiple mathematics models.Results Regression analysis of single factors showed that there are statistical negative correlation between HIV viral load and CD+_3 or CD+_4 cell counts(r=0.6013,P=0.0177;r=-0.8828,P=0.0000),and positive correlation between HCV viral loads and CD+_3 or CD+_4 cell counts(r=0.5931,P=0.0198;r=0.8627,P=0.0000),and that there are statistical negative correlation between HIV and HCV viral loads(r=-0.8954,P=0.0000).Analysis of multiple factors showed that there are statistical negative correlation between HIV viral loads and CD+_3 or CD+_4 cell counts or HCV viral loads (r=-0.6051,P=0.0169;r=-0.8828,P=0.0000;r=-0.8954,P=0.0000).Analysis of stepwise regression showed that CD+_4 cell counts and CD+_4/CD+_8 ratio and HCV viral loads have statistical negative correlation with HIV viral loads.Conclusion The interference and competitive inhibition are observed in viral loads of HIV and HCV in cases which are super-infected with HIV and HCV,and CD+_4 count are variously changed by showing a decresing tendency.
出处
《中国艾滋病性病》
CAS
2005年第2期84-87,共4页
Chinese Journal of Aids & STD
