摘要
为探讨Tet的抗炎作用及机制,在大鼠胸膜腔内注射Car复制胸膜炎。注射后2—48h,Neu-和ACC-PLA_2活性明显增强,胸膜渗出液量、蛋白质渗出量和白细胞游出数随之增加。Tet剂量依赖性地抑制上述各指标的变化,且Tet引起的PLA_2活性的降低与炎症指标的减少密切相关。提示Tet有良好抗炎作用,其机制可能涉及抑制PLA_2激活和释放。
A model of pleurisy was established with an intrapleural injection of car-rageenan (Car, 10 mg·kg-1) in rats to explore the anti-inflammatory action of tetrandrine (Tet) and its mechanism. After the injection of Car, the exudate, protein and neutrophils appeared in the pleural cavity of rats at 2 h, then increased progressively, and reached a peak level at 12 h and remained high up to 48 h. The phospholipase A2(PLA2) activity of neutrophils (Neu-PLA2) and of the acellular component (ACC-PLA2) in the pleural exudate intensified at 2 h, rose to maximal intensity at 8 h, and started to decline at 48 h. The changes of the amount of exudate, protein content and neutrophil count, and parameters indicating the inflammatory response, were closely related to that of the PLA2 activity (r=0. 749 - 0. 928, P<0. 05 or 0. 01). By ig gavage of Tet (10 - 80 mg·kg-1) to rats at 30 min before and 4 h after the injection of Car, the inflammatory parameters were reduced and the PLA2 activity was inhibited, dose-dependently. The reductions of the inflammatory parameters were significantly associated with the inhibition of the PLA2 activity (r = 0. 928-0. 993, P<0. 05 or 0.01).
These results indicate that Tet has a fine anti-inflammatory action and its mechanism may involve the inhibition on the activation and release of PLA2 of inflammatory cells.
出处
《中国药理学报》
CSCD
1994年第5期477-480,共4页
Acta Pharmacologica Sinica
基金
Project supported by the National Natural Science Foundation of China
№ 38970906.
关键词
粉防己碱
磷脂酶A类
抗炎作用
tetrandrine
carrageenan
pleural effusion
neutrophils
phospholipases A
