摘要
目的观察体外培养的成人毛囊隆起细胞的生长特性。方法采用一步消化法和显微分割技术获取毛囊隆起细胞,并进行培养、传代,连续观察细胞的生长状况、形态特征以及表达特性。结果原代培养的第2d便可见细胞生长,并表现出很强的增殖潜能,6d时达到最高,并维持数日;光镜下细胞小而圆,大小一致,可见有丝分裂相;免疫细胞化学染色可见K19阳性细胞,表达随培养时间延长呈下降趋势。结论体外培养的毛囊隆起细胞具有原始细胞特性,提示毛囊中潜在的干细胞定位于毛囊隆起。
Objective To observe the growth characteristic of bulge-originated cells from human hair follicles in vitro. Methods The bulges which were isolated from human hair follicles by dispase and microdissection were cultured. The morphological and biological characteristic of the cultured bulge cells were observed by light microscopy and immunocytochemistry (ICC). Results Proliferated cells could be observed in the second day after being inoculated. The number of these cells, with greater proliferation potential, reached the peak at the sixth day and maintain several days. In addition, the mitotic figures appeared and the cells behave the similar morphologic features, meanwhile the cells strongly expressed K19 and showed a descensive tendency in long-time growth.Conclusion The cultured bulge cells kept the primitive characteristic, which suggested that the putative follicle stem cells resided in the bulge area.;
出处
《解剖学报》
CAS
CSCD
北大核心
2005年第3期269-271,共3页
Acta Anatomica Sinica
基金
第三军医大学科研及创新基金(XG200338)
关键词
毛囊
隆起
生长
体外培养
免疫细胞化学
人
Hair follicle
Bulge
Growth
in vitro
Immunocytochemistry
Human
