摘要
采用制备型高效液相色谱法将菜籽油不皂化物分离为甾醇、４－甲基甾醇、三萜醇和烃四类。甾醇部分经乙酰化后，由气相色谱法分析，并采用硝酸银硅胶制备薄层色谱法和高效液相色谱法进一步分离。分得的主要组分经薄层、气相色谱、溶点、红外光谱和质谱鉴定，并分离得到β－谷甾醇，菜油甾醇，菜籽甾醇和△５－燕麦甾醇等纯品。被认定的有２４－亚甲基胆甾醇、豆甾醇、△７－豆甾烯醇和△７－燕麦甾醇。
The unsaponifiable matters of rape seed oil were divided into a sterol and three other fractions by HPLC. The acetates of sterols were further separated by TLC with silver nitrate impregnated silica gel G and HPLC. The ident.ification of the major individual components was carried out by TLC,OLC,melting point, IR spectrum and MS. β-sitosterol, campesterol, brassicasterol and △5-avenasterol in sterol fraction were isolated and identified, while trace 24 -methylenecholesterol, stigmasterol, △7-stigmasterol and △7-avenasterol were identified only.
出处
《中国油料》
CSCD
北大核心
1994年第1期29-32,共4页
关键词
菜籽油
不皂化物
甾醇
Rape seed oil
Unsaponifiable matter
Sterol
