摘要
目的获取蓝氏贾第虫谷氨酸脱氢酶(GDH)全基因,并对其进行序列分析。方法利用PCR技术扩增蓝氏贾第虫GDH,并将其插入pMD18-T载体,酶切鉴定后进行序列测定及分析。结果DNA序列分析表明,所克隆的GDH基因序列与蓝氏贾第虫Portland-1株谷氨酸脱氢酶基因基本一致,该基因5’上游区具有典型的CAAT/TATA盒,含有GDH启动子序列。结论本研究获得了蓝氏贾第虫GDH启动子,为贾第虫病毒的反向遗传学操作奠定了基础。
To obtain the total glutamate dehydrogenase(GDH) gene from Giardia lamblia, the gene was amplified by PCR and inserted into pMD18-T vector. The recombinant plasmid pMD/GD11 was identified by restriction enzyme digestion and sequencing. It was proved that the sequence of GDH gene was just the same as that of the Portland-1 strain of G.lamblia, and it had typical CAAT/TATA box in the up-stream of 5'terminal and contained sequences of the GDH gene promoter.It is evident that the GDH gene promoter was successfully obtained in the present study, thus providing foundation for the reverse genetics technique of GLV.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2005年第6期473-475,共3页
Chinese Journal of Zoonoses
基金
国家自然科学基金资助(30300260)
