摘要
用神方补肾生血药处理Ｗｉｓｔａｒ大鼠，发现有明显的抗衰老作用。用３Ｈ－甲基掺入法测定了鼠肝ＤＮＡ甲基化酶活力，发现药物处理可导致ＤＮＡ甲基化酶层析行为的改变，比活力增高。说明神方补肾生血药改变了大鼠肝ＤＮＡ甲基化酶的分子结构，从而影响其比活力，这说明改变ＤＮＡ甲基化酶，从而影响其ＤＮＡ甲基化水平，可能是该药物抗衰老作用的机制之一。
An obvious anti-aging effect was found by Bushen Shengxue drug to treat Wistar rats. Using a method about incorporation of 3H-labled methyl group of S-adenosyl-methionine (SAM)into DNA bases, the DNA methylase activity was assayed.The specific activity of DNA methylase was increased by treatment of this drug. The chromatographic behaviour of DNA methylase from rat liver was also changed.These results showed that the mechanism of anti-aging effect of this drug is probably related to DNA methylation.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1994年第6期354-356,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金
关键词
DNA
甲基化酶
抗衰老药
Bushen Shengxue drug, DNA methylase, Chromatography
