摘要
目的探讨肝细胞生长因子对牙髓细胞生长增殖的影响。方法以体外培养的牙髓细胞为实验对象,以不同浓度肝细胞生长因子作用于牙髓细胞,通过二甲基噻唑二苯基四唑溴盐比色法对细胞的增殖情况进行研究,用流式细胞术对细胞的周期进行分析。结果1～200μg L的肝细胞生长因子可明显促进牙髓细胞的增殖(P<0.05),100μg L为最大效应浓度。100μg L的肝细胞生长因子作用牙髓细胞3d对其细胞周期无显著性影响,作用5d缩短了G1期,增加了S期比例。结论肝细胞生长因子对牙髓细胞的增殖有促进作用。
Objective to study the effect of hepatocyte growth factor on the proliferation of dental pulp cell.Methods the 4th generation dental pulp cell cultured in vitro was used as target cell. 1～200 μg/L hepatocyte growth factor was added in the test group while the pure cell culture DMEM as control. The MTT method and flowcytometry were applied to assay the proliferation and cell cycle of dental pulp cell of different groups.Results 1～200 μg/L hepatocyte growth factor showed promoting effect to the proliferation of pulp cell since the 5th day (P<0.05). 100 μg/L was found to be the optimal concentration. Also on the 5th day, 100 μg/L hepatocyte growth factor decreased the G1 subcycle and increased the S subcycle of dental pulp cell (P<0.05). While on the 3rd day, it had no effect on the cell cycle.Conclusion hepatocyte growth factor had positive effect on the proliferation of dental pulp cell, with 100 μg/L as the optimal concentration.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2005年第3期198-200,共3页
West China Journal of Stomatology
关键词
肝细胞生长因子
牙髓细胞
细胞增殖
细胞周期
hepatocyte growth factor
dental pulp cell
cell proliferation
cell cycle
