摘要
利用RAPD和酯酶同工酶技术对来自国内外的10个灵芝属代表菌株进行了遗传多样性分析。RAPD分析结果表明,在0.560的相似性水平上分成3个组:第1组包括密纹薄芝(1号)、两个灵芝(3号和4号)和两个无柄灵芝菌株(7号和8号);第2组包括灵芝(2号)、近拟鹿角灵芝(5号和6号)和紫芝(10号);第3组是树舌(9号)。这一结论与传统分类学结论基本一致。当相似性水平达到0.800时,上述10个菌株聚成8组,这与传统分类学中种的分类几乎一致。酯酶同工酶的分析结果表明,在0.560的相似性水平上,所有菌株分为两组:第一组是树舌(9号);其他菌株构成一组。这一结论与传统分类学结论也一致。当相似性水平达到0.800时,上述菌株分为7组:其中3号、4号、7号和8号构成一组,其余的同RAPD结果。比较两种方法所得结果发现,在较高的相似性水平(0.840)上,它们的结论是一致的。这表明,RAPD和酯酶同工酶技术在灵芝种间鉴定时是有效的,甚至RAPD在种内鉴定时都是有效的。
Genetic diversity among 10 representative strains of Ganoderma from different provinces of China and other countries was analyzed by RAPD and esterase isozyme techniques,respectively.The dendrogram derived from RAPD data indicated that they could be divided into three distinct groups at the similarity level of 0.560:the first group included Ganoderma tenue,G.resinaceum,G.lucidum Xinzhou and G.lucidum;the second one included G.spp.,G.subamboinense and G.sinense;and the third group contained only G.applanatum.The result was in near accordance with that of traditional classification of Ganoderma.However these 10 strains could be divided into 8 groups at the similarity level of 0.800,and the result was in near accordance with that of traditional classification.The result inferred from the method of esterase isozyme indicated that these strains could be divided into two distinct groups at the similarity level of 0.560:the first group composed of G.applanatum;the second one included 9 other strains.The result was also in accordance with that of traditional classification.And G.resinaceum,G.lucidum Xinzhou and G.lucidum composed one group and other groups were the same as those inferred from RAPD at the similarity level of 0.800.Results inferred from these two methods were in accordance with each other at the high similarity level of 0.840.The present study indicated that RAPD and esterase isozyme techniques were both effective for classification of the species in Ganoderma,and RAPD was even effective in differentiating strains of the same species.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2005年第2期133-136,共4页
Journal of Nanjing Agricultural University
基金
上海市农委科技兴农重点攻关项目
