摘要
[目的]建立C3、C4测定法用于血液免疫反应路线图研究。[方法]癌细胞(S180,5×106/ml)或生理盐水0.2ml加入新鲜抗凝(枸橼酸抗凝)全血细胞(或白细胞0.2ml)和0.3ml血浆中,37℃温浴1h。C3、C4测定采用全自动特种蛋白分析仪检测。[结果]癌细胞可激活血液免疫反应,全血细胞加补体组C3和C4含量高于白细胞加补体组。[结论]补体和红细胞在血液免疫反应路线图中具有重要的增强作用。
Purpose] To establish method for detecting C3 and C4 in the study of hemoimmune reaction road map. [Methods] Cancer cells(S180, 5×106/ml) or 0.2ml NS were added in fresh anticoagalant(by citric acid) whole blood cells 0.2ml(or white blood cells 0.2ml) and 0.3ml plasma, and incubated for 1 hour at 37℃. C3, C4 were detected by BNProSpect of DADE Behring. [Results] Cancer cells activitated hemoimmune reaction and level of C3 and C4 in whole blood cells was higher than those in white blood cells. [Conclusion] The complement and red blood cells play enhaning effect on hemoimmune reaction.
出处
《肿瘤学杂志》
CAS
2005年第3期167-168,共2页
Journal of Chinese Oncology
