摘要
目的:探讨内质网应激反应对于诱导病毒性心肌炎心肌细胞凋亡的作用。方法:120只小鼠随机分成实验组和对照组实验组腹腔感染柯萨奇病毒B3(CVB3),分别于1、2、4周取心脏标本,用TUNEL法检测心肌细胞凋亡,RT-PCR检测心肌细胞内质网伴侣蛋白GRP78和GRP94的mRNA表达水平。结果:实验组存活小鼠内质网伴侣蛋白GRP78和GRP94的mRN表达水平均明显高于对照组(P<0.01,P<0.05),并且其变化趋势与心肌细胞凋亡数相似。结论:内质网应激反应可能对于诱导病毒性心肌炎的心肌细胞凋亡有一定作用。
Objective:To investigate the role of endoplasmic reticulum stress in induction of cardiac myocyte apoptosis in viral myocarditis.Methods:One hundred and twenty mice were divided randomly into the experimental group and the control group. The insitu end-labeled DNA (TUNEL) was used to analyze cardiomyocyte apoptosis, and the expression of endoplasmic reticulum chaperones GRP78 and GRP94 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR) in mice inoculated with coxsackievirus B3 (CVB3) after 1, 2 and 4 weeks.Result:The expression of GRP78 and GRP94 were increased significantly in the infected group compared with the control group. The tendency of change of GRP78 and GRP94 was similar to the change of cell apoptosis.Conclusion:These findings suggest endoplasmic reticulum stress may contribute to the cardiac myocyte apoptosis in viral myocarditis.
出处
《广西医科大学学报》
CAS
北大核心
2005年第2期157-159,共3页
Journal of Guangxi Medical University
基金
广西壮族自治区科委自然科学基金课题(桂科基0448048)
关键词
内质网应激
伴侣蛋白
柯萨奇病毒B组
心肌炎
凋亡
endoplasmic reticulum stress
chaperone
coxsackievirus B
myocarditis
apoptosis
