摘要
目的探讨由牛磺酸、丹参组合而成的复方心康口服液,对大鼠心肌细胞急性缺氧/复氧(A/R)损伤的保护作用。方法以1￣3d的SD大鼠的心室肌细胞为基质,利用模拟缺氧(缺血)溶液和模拟复氧(再灌)溶液,建立心肌细胞缺氧/复氧损伤模型,分为对照组(复氧组)、A/R组、心康组,分别观察3组的心肌细胞存活率、培养液中乳酸脱氢酶(LDH)活性、心肌细胞超微结构。结果A/R组与对照组相比其细胞存活率显著降低(P<0.01),心康组与A/R组相比细胞存活率明显升高(P<0.01);A/R组与对照组相比其LDH活力显著升高(P<0.01),心康组与A/R组相比LDH活力明显降低(P<0.01);对照组心肌细胞超微结构正常,A/R组心肌细胞超微结构破坏,成不可逆损伤,心康组细胞超微结构大有改善。结论复方心康口服液对大鼠心肌细胞急性A/R损伤具有显著的保护作用,表现为心肌细胞存活率明显增加、LDH活性显著降低及改善心肌细胞超微结构。由此可见,复方心康口服液作为心肌缺血、再灌注损伤治疗的辅助性用药具有较好的临床应用前景。
Objective To explore the protective effect of Xinkang oral liquid made from taurine and radix salviae miltiorrhizae on the myocardium-experienced acute anoxia/reperfusion injury. Methods The ventricular myocardial cells of 1~3 days old SD rats were divided into three groups.In control group,the cells were cultivated with reperfusion liquid.In A/R group,the cells were cultivated with anoxia/reperfusion liquid.In Xinkang group,the cells were firstly cultivated with Xinkang oral liquid and secondly with anoxia/reperfusion liquid.Myocardial survival ratio,lactate dehydrogenase(LDH) activity in culture fluid and myocardial ultrastructure were observed. Results Myocardial survival ratio of group A/R was significantly lower than that of control group(P<0.01).The ratio of group Xinkang was significantly higher than that of group A/R(P<0.01).LDH activity of group A/R was significantly higer than that of control group(P<0.01).The activity of group Xinkang was significantly lower than that of group A/R(P<0.01).Myocardial ultrastructure of control group was normal,and that of group A/R was seriously destroyed.The structure of group Xinkang was significantls improved. Conclusion Compound Xinkang oral liquid can protect the myocardium-experienced acute anoxia/reperfusion injury through increasing myocardial survival ratio,decreasing LDH activity and improving myocardial ultrastructure.There is excellent prospect of applying compound Xinkang oral liquid to treat myocardial anoxia/reperfusion injury.
出处
《中国药物与临床》
CAS
2005年第6期436-438,共3页
Chinese Remedies & Clinics
基金
深圳市科技计划资助项目(20024149)
