摘要
作者应用聚合酶链反应（ＰＣＲ）检测ｅ系统阳性的慢件乙型肝炎（乙肝）患者血清乙肝病毒（ＨＢＶ）ＤＮＡ，筛选出乙肝ｅ抗原（ＨＢｅＡｇ）阳性／乙肝ｅ抗体（抗—ＨＢｅ）阴性和ＨＢｅＡｇ阴性／抗—ＨＢｅ阳性各２０份ＰＣＲ阳性血清，分别用人工合成的ＨＢＶ前Ｃ区基因寡核苷酸探针Ｍ０（无突变），Ｍ１（１点突变），Ｍ２（２点突变）进行斑点杂交，结果发现ＨＢｅＡｇ阴性／抗—ＨＢｅ附性慢性乙肝患含有１７例（占８５％）在１８９６位核苷酸发生点突变，形成终止密码，其中１０例（占５０％）发生２点突变。选突变ＰＣＲ产物直接测序，同样证实点突变的存在。提示乙肝患含ＨＢｅＡｇ阳性自然转换为抗—ＨＢｅ阳性、并不一定是病毒复制减弱，而可能足ＨＢＶＤＮＡ前Ｃ区发生了基因变异。
e examined the serum HBV DNA of the chron-ic hepatitis B
patiens with positive c-system by usingpolymerase chain reaction (PCR). Twenty samples
ofHBeAg(+) / anti-HBe(-) postitive PCR products and20 samples of HBeAg(-) / anti-HBe(+)
positive PCRproducts were selected. These produets weredot-hybridized by synthetic HBV
pre-C region gencoligonucleotide probes MO (non mutated), M1 (one)point-mutated), M2 (two
point-mutatied). The re-sults thus obtained showed that for 85% (17 / 20) ofthe chronic hepatitis
patients with HBeAg(-)/ anti-HBe(+), point mutation occurred at the 1 896thnucleolide, resulting in
the stop codon, and half( 10 / 20) of the patients suffered two points mutation.The direct
determination of the sequence of some mu-tation PCR products selected proved the existence
ofpoint mutation. The result indicated that the naturalconversion of hepatitis patients' HBeAg(+)
toanti-HBe(+) may have resulted from thegenovariation in the pre-c region of HBVDNA ratherthan
from the decreased of virus replication.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1994年第3期144-146,共3页
National Medical Journal of China
关键词
乙型肝炎病毒
基因
聚合酸链反应
Hepatitis B virus Gene, virusPolymerase
chain reaction
