摘要
目的研究p27kip1和bcl-2在增生性子宫内膜及子宫内膜癌组织中的表达以及与临床病理参数的关系。方法采用免疫组化S-P法检测p27kip1和bcl-2在24例子宫内膜(单纯增生+复杂增生)、20例子宫内膜不典型增生和56例子宫内膜癌组织中的表达。结果1·p27kip1在(单纯增生+复杂增生)组、内膜不典型增生组、子宫内膜癌组中的表达率无显著性差异(P>0·05)。bcl-2阳性表达在(单纯增生+复杂增生)组与内膜不典型增生组、内膜癌组相比均具有显著性差异(P<0·05),内膜不典型增生组与内膜癌组相比差异无显著性(P>0·05)。2·p27kip1表达与子宫内膜癌临床分期,组织学分化程度和肌层浸润程度有关,与病理类型及淋巴转移无关;bcl-2表达高低与淋巴转移有关,与其他临床病理参数无关。3·p27与bcl-2的表达呈正相关(P<0·05)。结论p27kip1及bcl-2在子宫内膜癌发生、发展的不同时期起作用,bcl-2可作为子宫内膜由良性病变向恶性病变进展的早期判断指标,p27kipl表达降低与子宫内膜癌的恶性进展有关,可作为判定内膜癌预后的指标。联合检测p27和bcl-2对早期诊断子宫内膜癌、确定肿瘤恶性程度、预测预后有重要作用。
Objective To study the expression of p27 and bcl-2 in the endometrial hyperplasia, endometrial carcinoma and the relationship between the expression and the clinic pathological parameter. Methods S-P method was used to examine the expression of the p27 and bcl-2 in the 24 endometrial(simple and complex) hyperplasia, 20 endometrial atypical hyperplasia and 56 endometrial carcinoma. Results 1.the expression of p27 was no significance difference among the three sets(P>0.05). to match with atypical hyperplasia and endometrial carcinoma, the expression of bcl-2 in the simple hyperplasia and complex hyperplasia has a significant difference(P<0.05), there was no significance difference between atypical hyperplasia and endometrial carcinoma(P>0.05). 2.the expression of p27 is related with the clinical stage, histological grade and muscle invasion(P<0.05), and the expression is not related with pathological type and lymphnode metastasis(P>0.05), the expression of bcl-2 is related to lymphnode metastasis(P<0.05) and not related with other clinic pathological parameter(P>0.05). 3.the expression of p27 and bcl-2 is positive correlated(P<0.05). Conclusion p27 and bcl-2 play roles in different stage of the development of the endometrial carcinoma. bcl-2 may predicting the occurrence of the endometrial carcinoma in the early stage, the low expression of p27 is associated with the development of endometrial carcinoma and may be regarded as a useful index for prognosis. together inspected p27and bcl-2 has important effect in diagnosing early endometrial cacinoma, comfirming degree of malignency and forcasting prognosis.
出处
《中国实验诊断学》
2005年第3期380-383,共4页
Chinese Journal of Laboratory Diagnosis
