摘要
目的探讨孕妇巨细胞病毒感染对胎儿的影响。方法采用ELISA和PCR法,对280例孕妇的血清同时进行检测,ELISA检测HCMV-IgM、PCR法检测HCMV-DNA。结果1·HCMV-IgM阳性17例,阳性率为6·07%。PCR法检测血清HCMV-DNA,阳性44例,阳性率为15·71%。ELISA法和PCR法检测同时阳性为6例,280例孕妇HCMV感染阳性共计55例,阳性率为19·64%。2·对55例HCMV感染(+)孕妇和27例感染(-)孕妇的胎儿脐血应用ELISA和PCR法检测,观察孕妇的妊娠结果,追踪HCMV感染孕妇的婴儿近期发育情况。55例HCMV感染(+)孕妇的胎儿脐血HCMV-IgM和HCMV-DNA阳性14例,阳性率即为宫内传播率,为25·45%。27例感染(-)孕妇的胎儿脐血HCMV-IgM,HCMV-DNA均阴性。对HCMV感染阳性及阴性的母儿HCMV感染情况相关观察,有显著性差异。3·14例先天感染儿中畸胎2例,1例为小头畸形,另1例唇裂。4·随访HCMV感染(+)孕妇的婴儿31例中,3例感染儿发育异常,异常率为9·67%,27例无先天感染儿随访中未见异常,先天感染儿发育异常显著高于无先天感染儿(P<0·005)。结论HCMV感染在本地区的感染较严重,对计划生育中的优生优育影响较大,本文结果为提高预防HCMV感染有积极的指导价值。
Objective cytomegalovirus(cmv) is the most common cause of intrauterine infection In order to study the effects of fetal development after pregnant women's HCMV infection. Methods This essay used ELISA and PCR to screen for serum in 280 pregnantwomen. ELISA serum HCMV-IgM, PCR screene dforserum HCMV-DNA. Results HCMV-IgM positiv ecases were 17 in 280 pregnant women, positiverate was 6.07%, HCMV-DNA positive cases were 19.64%. We also detected fetal umbilical blood of 55 HCMV infectied. Mothers andthat of 27 uninfected oneswith ELISA and PCR. At the same time, WE observed the pregnant result and the fetal development after mothers intrauterine HCMV infection. positive cases of fetal umbilical blood HCMV-IgM and HCMV-DAN in55HCMV infected pregnant women were 14, positive rate or interuterine transmission rate was 25%. but that in 27 uninfected pregnant women was allnegative, By studying 82(55 infected and 27 uninfetuses 'HCMV infection, We found there were) significant difference(P<0.05). In 14 congenital infected fetuses whose mothes were the other was harelip. In 31 fetuses whose mothers were HCMV infected, 3 cases were abnormal development, abnormal rate was 9.67%, but there was no fetal malformation in 17 uninfescted fetuses, Malformation in congenital infected fetuses was higher than that in uninfected fetuses(P<0.05). We can concluded that HCMV infection was serous in our district, so it had animportant effection leugenic in family planning. Conclusion Theresult of this essay will have a positive directive function to raise and take precaution against HCMV infection.
出处
《中国实验诊断学》
2005年第3期340-343,共4页
Chinese Journal of Laboratory Diagnosis
