摘要
目的观察汉防己甲素对增生性瘢痕来源的成纤维细胞的MMP-1mRNA表达的影响。方法培养增生性瘢痕来源的成纤维细胞,然后分别按终浓度为1、5、10mg/L加入汉防己甲素,继续培养24h,提取细胞内的RNA,经RT-PCR获得cDNA,再用MMP-1的特异性引物,通过PCR扩增,得到MMP-1的mRNA,并以β-actin为内参照物,进行琼脂糖凝胶电泳,最后用Band-scan扫描系统测定各产物密度值,用凝胶自动成像仪拍照。结果在汉防己甲素的作用下,成纤维细胞的MMP-1mRNA表达增加。结论汉防己甲素可使增生性瘢痕来源的成纤维细胞的MMP-1的mRNA转录增加,从而增加了MMP-1的合成,因而可能对增生性瘢痕具有预防和治疗作用。
Objective To observe the effects of tetrandrine on the expression of MMP-1 mRNA of fibroblasts derived from hypertrophic scar. Methods The fibroblasts derived from hypertrophic scar were harvested for 24 hours after addition of tetrandrine and cellular RNA was extracted. Total cellular RNA was used for getting cDNA by RT-PCR. PCR amplifications were performed with a pair of primers specific to MMP-1 and β-actin. PCR products for MMP-1 and β-actin were separated by a 2% agarose gel and stained with ethidium bromide. The intensity of the bands was quantified with an image computer software package. Values were normalized to the mRNA level of β-actin. Results The data indicated that the expression of MMP-1 mRNA of fibroblasts derived from hypertrophic scar were enhanced by tetrandrine. Conclusion MMP-1 plays an important role in the wound healing of burn injury and hypertrophic scar formation. Since tetrandrine can enhance the expression of MMP-1 mRNA produced by hypertrophic scar fibroblast, tetrandrine effective in prevention and the treatment of hypertrophic scar.
