摘要
目的:探讨不同部位残胃黏膜幽门螺旋杆菌(Helicobacterpylori,Hp)感染与IL8mRNA表达的关系。方法:对58例残胃患者进行胃镜检查,于吻合口和胃体大弯处,采用两点取材法取材进行胃镜活检。活检标本Hp的感染采用HE和Giemsa染色法检查。活检标本中IL8mRNA表达水平采用实时半定量PCR法检测。结果:58例残胃患者中,38例感染Hp(65.5%)。34例以BI式吻合者中,残胃体部Hp感染阳性者IL8mRNA表达的水平,明显高于Hp感染阴性者的表达水平,分别为0.11±0.07和0.02±0.01(P<0.05);24例BII式吻合者,无论是残胃体部还是吻合口处,Hp感染阳性者IL8mRNA表达的水平,均明显高于Hp感染阴性者的表达水平(吻合口处IL8mRNA表达为:0.32±0.24和0.02±0.01;残胃体部为:0.18±0.08和0.01±0.01)(P<0.05)。结论:残胃黏膜Hp的感染可诱导IL8mRNA表达。残胃体部IL8mRNA的表达主要与Hp感染有关;吻合口处IL8mRNA表达,除与Hp感染有关外,也受胆汁反流影响。
AIM: To explore the relationship between helicobacter pylori (Hp)infection and expression of interleukin-8 (IL-8)in the remnant stomach mucosa. METHODS: Fifty-eight patients with gastrectomy were examined by upper gastrointestinal endoscopy. Biopsy specimens were obtained from stoma and the greater curvature of the upper corpus to detect Hp infection (by H.E. and Giemsa staining) and IL-8 expression (by real-time semi-quantitative RT-PCR). RESULTS: Hp infection was detected in 65.5% (38/58)of the remnant stomach mucosa. In 34 patients with Billroth I (BI) anastomosis, IL-8 in corpus was significantly higher in Hp-positive patients than in Hp-negative patients(0.11±0.07 vs 0.02±0.01, P<0.05). In 24 patients with Billroth II (BII) anastomosis, IL-8 in stoma and corpus in Hp-positive patients was significantly higher than in Hp-negative patients (P<0.05). CONCLUSION: Hp infection induces IL-8 expression in remnant stomach mucosa. In corpus, IL-8 mRNA expression is mainly related with Hp infection, while in stoma, IL-8 mRNA expressions may be related with bile reflux besides Hp infection.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2005年第4期503-505,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家"十五"科技攻关计划课题(No.2001BA703B06B)
关键词
残胃
幽门螺旋杆菌
IL-8
remnant stomach
helicobacter pylori
interleukin-8
