摘要
用线性扫描伏安法研究了在pH3.0的Britton Robinson(B R)缓冲溶液中变色酸2B与蛋白质反应前后的电化学变化。在酸性溶液中变色酸2B在-0 48V(vs.SCE)处有一个灵敏的伏安还原峰,当在溶液中加入人血清白蛋白后能够发生相互作用形成生物超分子复合物,使变色酸2B的伏安还原峰峰电流下降而峰电位基本保持不变。优化了结合反应条件和电化学测定条件,考察了常见干扰物质对测定的影响。在最佳条件下,峰电流的下降值同人血清白蛋白(HSA)的浓度在2.0～25.0mg L范围内呈良好的线性关系,线性回归方程为Δip″(nA)=50.56ρ(mg L)-6.72,相关系数r为0.995,检出限为1.84mg L。将该方法应用于实际人血清样品的测定,结果与经典的考马斯亮蓝G 250光度法一致,回收率令人满意。
In pH 3.0 Britton-Robinson (B-R) buffer solution chromotrope 2B may interact with protein to form a supermolecular complex. Chromotrope 2B is an azo compound and has a sensitive reduction peak at -0.48 V (vs. SCE) on the hanging mercury drop electrode. After adding human serum albumin (HSA) to above solution, the reduction peak current of chromotrope 2B decreases linearly with HSA concentration from 2.0 to 25.0 mg/L, the linear regression equation Δi_p″(nA)=50.56ρ ((mg/L))-6.72,(γ=0.995)and the detection limit of 1.84 mg/L. This method is further applied to determining the content of HSA in blood samples and the results are in good agreement with the traditional Coomassie brilliant blue G-250 spectrophotometric method.
出处
《分析试验室》
CAS
CSCD
北大核心
2005年第5期59-62,共4页
Chinese Journal of Analysis Laboratory
基金
中国博士后科学基金(2003033492)
国家自然科学基金(20405008
20375020)资助项目
