摘要
目的:探讨细粒棘球蚴病宿主体内T淋巴细胞亚群在免疫调节和发病中的作用。方法:对感染细粒棘球蚴的昆明种小鼠观察20周,在不同时间取脾制备细胞悬液,用流式细胞术(FCM)检测CD4+、CD8+淋巴细胞的百分率。结果:细粒棘球蚴感染后的小鼠,于2周时CD4+、CD8+淋巴细胞的百分率明显增高(P<0.01),随后CD4+的百分率逐渐降低,CD8+的百分率逐渐增高,至感染16周后,CD4+的百分率较正常对照组下降(P<0.01),CD8+的百分率较正常对照组升高(P<0.01),CD4+/CD8+比值降低(P<0.01)。结论:感染细粒棘球蚴的小鼠早期以CD4+细胞反应为主,具有保护性免疫,感染后期逐渐以CD8+细胞为主,使机体呈免疫抑制状态,有利于细粒棘球蚴增殖。
Objective: To explore the immunological regulation mechanism of mice infected with Echinococcus granulosus. Methods: The course of the infection was observed for 20 weeks. Spleen cells from mice infected with Echinococcus granulosus were collected in different period, CD4+ and CD8+ cell levels were determined by flow cytometre. Result: Two weeks after infection,the CD4+ and CD8+ cell levels showed a significant increase(P<0.01), then CD4+ cell decreased and CD8+ cell increased gradually. Since 16 weeks after infection,the percentage of CD4+ cell were higher than that of control group(P<0.01)and the percentage of CD8+ cell were lower than that of control group(P<0.01). There was a significant drop down of CD4+ CD8+ ratio since 16 weeks after infection(P<0.01). Conclusion: CD4+ cell is mainly response in the early stage of infection, CD4+ response is replaced by CD8+ response in later period of infection. CE host exhibits an immune inhibition response,which is very useful to hosts with Echinococcus granulosus infection.
出处
《新疆医科大学学报》
CAS
2005年第5期402-404,共3页
Journal of Xinjiang Medical University
