摘要
【目的】探讨大蒜素对人膀胱癌细胞株BIU87细胞凋亡的影响。【方法】MTT法检测大蒜素对BIU87细胞生长的影响;倒置显微镜、TUNEL法观察细胞形态变化,流式细胞仪检测细胞凋亡率和细胞周期的变化;半定量RT-PCR法检测凋亡相关基因bcl-2,bax,caspase-3mRNA表达的变化,比色法测定caspase-3活性变化。【结果】大蒜素能明显抑制BIU87细胞的生长,与大蒜素的浓度和作用时间有关,5mg/L大蒜素作用5d可以杀死50%以上的细胞;可诱导BIU87细胞凋亡,细胞周期阻滞在S-和G2期;凋亡相关基因bcl-2表达减少,bax无明显变化,caspase-3表达及活性增高。【结论】诱导细胞凋亡是大蒜素发挥抗瘤效果的一种重要机制;凋亡发生可能与细胞周期阻滞、bcl-2和caspase-3基因表达及活性变化有关。
[Objective] To study the mechanism of allitridi-induced apoptosis in BIU87 cells.[Methods] Growth inhibition were estimated using MTT assay. Apoptotic cells were detected by in-situ cell apoptosis detection kit, and confirmed by flow cytometry. The mRNA level affected by allitridi was determined by RT-PCR. Caspase-3 activity was estimated with chromometry. [Results] Allitridi inhibited the proliferation of BIU87 cells and induced apoptosis, S-phase and G2 -phase arrest was induced. There were apparent changes of bcl-2, caspase-3 mRNA and a rise of caspase-3 activity; while no changes of bax was found. [Conclusion]Allitridi can inhibit the proliferation of BIU87 cells by inducting apoptosis, which may be through S-and G2-phase arrest, down-regulation of bcl-2, and increasing caspase-3 expression and its activity.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2005年第4期432-434,438,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省自然科学基金面上项目(010719)
